Multi-Arm Polymeric Prodrug Conjugates of Cabazitaxel-Based Compounds

ABSTRACT

Among other aspects, provided herein are multi-arm polymeric prodrug conjugates of cabazitaxel-based compounds. Methods of preparing such conjugates as well as methods of administering the conjugates are also provided. Upon administration to a patient, release of the cabazitaxel-based compound is achieved.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims the benefit of priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application Ser. No. 61/426,161, filed on Dec. 22, 2010, the disclosure of which is incorporated herein by reference.

FIELD

This disclosure relates generally to conjugates of a cabazitaxel-based compound conjugated to a multi-arm, water-soluble polymer. The linkage between the cabazitaxel-based compound and the multi-arm, water-soluble polymer is releasable, thereby enabling release of the cabazitaxel-based compound following administration of the conjugate to a patient. The invention relates to and/or has application(s) in (among others) the fields of drug discovery, pharmacotherapy, physiology, organic chemistry and polymer chemistry.

BACKGROUND

Over the years, numerous methods have been proposed for improving the delivery of biologically active agents, particularly small molecule drugs. Challenges associated with the formulation and delivery of pharmaceutical agents can include poor aqueous solubility of the pharmaceutical agent, toxicity, low bioavailability, instability, and rapid in-vivo degradation. Although many approaches have been devised for improving the delivery of pharmaceutical agents, no single approach is without its drawbacks. For instance, commonly employed drug delivery approaches aimed at solving or at least ameliorating one or more of these challenges include drug encapsulation (such as in a liposome, polymer matrix, or unimolecular micelle), covalent attachment to a water-soluble polymer (i.e., conjugation) such as polyethylene glycol (i.e., PEG or PEGylation), use of gene targeting agents, and the like.

PEGylation has been employed to improve the bioavailability and ease of formulation of small molecule therapeutics having poor aqueous solubilities. For instance, water-soluble polymers such as PEG have been covalently attached to artilinic acid to improve its aqueous solubility. See U.S. Pat. No. 6,461,603. Similarly, PEG has been covalently attached to triazine-based compounds such as trimelamol to improve their solubility in water and enhance their chemical stability. See International Patent Application Publication No. WO 02/043772. Covalent attachment of PEG to bisindolyl maleimides has been employed to improve poor bioavailability of such compounds due to low aqueous solubility. See International Patent Application Publication No. WO 03/037384. Polymer conjugates of non-steroidal anti-inflammatory drugs (NSAIDs) and of opioid antagonists have also been prepared. See U.S. Patent Application Publication Nos. 2007/0025956 and 2006/0105046, respectively. Prodrugs of camptothecin having one or two molecules of camptothecin covalently attached to a linear polyethylene glycol have also been prepared. See U.S. Pat. No. 5,880,131. Prodrugs of irinotecan and docetaxel having (among other things) four molecules of drug covalently attached to a multi-arm polymer have been described in U.S. Pat. No. 7,744,861 and International Patent Application Publication No. WO 10/019,233, respectively.

Certain drugs, such as the alkaloids, are notoriously difficult to solubilize. Such alkaloids include the taxanes. Cabazitaxel is a drug within the taxane class of anti-cancer agents that is approved in combination with prednisone for the treatment of individuals suffering from hormone-refractory metastatic prostate cancer who are were previously treated with a docetaxel-containing treatment regimen.

The chemical name of cabazitaxel is (2α,5β,7β,10β,13α-4-acetoxy-13-({(2R,3S)-3-[(tertbutoxycarbonyl)amino]-2-hydroxy-3-phenylpropanoyl}oxy)-1-hydroxy-7,10-dimethoxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate, which has the following chemical structure:

Commercially, cabazitaxel in the form of an acetone solvate is available as an active constituent of pharmaceutical formulation under the JEVTANA® brand from Sanofi-aventis (Bridgewater, N.J.). This commercially available form also includes Polysorbate 80 as a solublizing agent for the drug.

Cabazitaxel is extensively metabolized in the liver (>95%), mainly by the CYP3A4/5 isozyme. Cabazitaxel is the main circulating moiety in human plasma, accompanied by seven metabolites that include three active metabolites (shown below as “Metabolite A,” “Metabolite B” and “Metabolite C”) derived from O-demethylation:

Although shown to provide several advantages over other taxanes (such as docetaxel) for certain indications, cabazitaxel is also associated with drawbacks as well. For example, cabazitaxel has a toxicity profile that is similar to docetaxel and is believed to be dependent on both the rate of drug clearance from the body as well as the patient's cytochrome CYP3A4 metabolic activity. In this regard, patients given a standard dose of cabazitaxel exhibit a wide interpatient variation in clearance and toxic effects, thereby supporting the concept that interpatient differences in metabolic activity accounts for at least some of this variance.

In addition, administration of cabazitaxel and other conventional taxane-based therapeutics may not distribute to the desired areas in vivo. In this regard, these conventional molecules may distribute relatively evenly throughout a patient's body, thereby exerting their effects on both normal and cancerous tissues. It would be desirous, however, if cabazitaxel and other conventional taxane-based could be modified in such a way so as to accumulate in tumor tissues while still retaining their potent anti-cancer effects.

The present invention seeks to address these and/or other needs.

SUMMARY

In one or more embodiments of the invention, conjugates are provided, the conjugates having a structure encompassed by the formula:

wherein:

R is a residue of a polyol, polythiol or polyamine bearing at from 3 to about 50 hydroxyl, thiol or amino groups;

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each Q is a linker (and, in one or more embodiments, a hydrolytically stable linker);

each POLY¹ is a water-soluble, non-peptidic polymer;

each X is spacer moiety that optionally includes a releasable linkage (e.g., a hydrolyzable linkage, an enzymatically degradable linkage, and so forth); and

q is a positive integer from 3 to about 50 (e.g., 4),

and pharmaceutically acceptable salts and solvates thereof.

In one or more embodiments of the invention, a conjugate-containing composition is provided, the conjugate-containing composition comprising four-arm conjugates, wherein at least 80% of the four-arm conjugates in the composition have a structure encompassed by the formula,

wherein:

R is a residue of a polyol, polythiol or polyamine bearing at from 3 to about 50 hydroxyl, thiol or amino groups;

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each Q is a linker (and, in one or more embodiments, a hydrolytically stable linker);

each POLY¹ is a water-soluble, non-peptidic polymer;

each X is spacer moiety that optionally includes a releasable linkage (e.g., a hydrolyzable linkage, an enzymatically degradable linkage, and so forth); and

q is a positive integer from 3 to about 50 (e.g., 4),

and pharmaceutically acceptable salts and solvates thereof.

In one or more embodiments of the invention, conjugates are provided, the conjugates having a structure encompassed by the formula:

wherein:

each R³ is selected from the group consisting of H, CH₃, CH(CH₃)₂, CH₂CH(CH₃)₂ and C(H)(CH₃)CH₂CH₃;

each n is a positive integer from 10 to about 400;

and pharmaceutically acceptable salts and solvates thereof.

In one or more embodiments of the invention, conjugates are provided, the conjugates having a structure encompassed by the following formula:

wherein:

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each n is a positive integer from 10 to about 400;

each m is a positive integer from 1 to about 12; and

and pharmaceutically acceptable salts and solvates thereof.

In one or more embodiments of the invention, conjugates are provided, the conjugates having a structure encompassed by the following formula:

wherein:

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each n is a positive integer from 10 to about 400;

each L¹ is —(CH₂)₀₋₆C(═O)— (for clarity, the carbonyl carbon is proximal to L²);

each L² is —NH(CH₂CH₂O)₁₋₁₀(CHR⁵)₁₋₆—, wherein each R⁵ is independently selected from the group consisting of H and lower alkyl (for clarity, the nitrogen is proximal to L¹); and

q is a positive integer from 3 to about 50 (e.g., 4),

and pharmaceutically acceptable salts and solvates thereof.

In one or more embodiments of the invention, conjugates are provided, the conjugates having a structure encompassed by the following formula:

wherein:

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each n is a positive integer from 10 to about 400;

each L¹ is —(CH₂)₀₋₆C(═O)— (for clarity, the carbonyl carbon is proximal to L³); and

each L³ is —[NH(CHR⁵)₁₋₆C(═O)]₁₋₄—) wherein each R⁵ is independently selected from the group consisting of H and lower alkyl (for clarity, the nitrogen is proximal to L¹); and

q is a positive integer from 3 to about 50 (e.g., 4),

and pharmaceutically acceptable salts and solvates thereof.

In one or more embodiments of the invention, conjugates are provided, the conjugates having a structure encompassed by the following formula:

wherein:

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each n is a positive integer from 10 to about 400;

each L¹ is —C(═O)— (for clarity, the carbonyl carbon is proximal to L³);

each L³ is —[NH(CHR⁵)₁₋₆C(═O)]₁₋₄—, wherein each R⁵ is independently selected from the group consisting of H and lower alkyl (for clarity, the nitrogen is proximal to L¹); and

q is a positive integer from 3 to about 50 (e.g., 4),

and pharmaceutically acceptable salts and solvates thereof.

In one or more embodiments of the invention, a pharmaceutical composition is provided, the pharmaceutical composition comprising a conjugate as described herein and a pharmaceutically acceptable carrier.

In one or more embodiments of the invention, a method is provided, the method comprising administering a conjugate as described herein (preferably in a pharmaceutical composition containing a pharmaceutically acceptable amount of the conjugate) to an individual.

In one or more embodiments of the invention, a method is provided, the method comprising a multi-arm in water-soluble, non-peptidic polymer structure having “q” polymer anus, each individual polymer arm having a reactive carboxylic acid group or activated ester thereof at its terminus, with “q” moles or greater of a compound having the following structure:

wherein:

R¹ is H or methyl;

R² is H or methyl, with the provisio that R¹ and R² are not both H; and

G is H or C(O)—(CH₂)₁₋₆—NH₂.

Additional embodiments of the present conjugates, compositions, methods, and the like will be apparent from the description that follows. Additional aspects and advantages of the present invention are set forth in the following description and claims, particularly when considered in conjunction with the accompanying examples and drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a plot showing the body weight changes (based on percent) of athymic mice following administration of 20K conjugates as part of a maximum tolerated dose study described in Example 15.

FIG. 2 is a plot showing the body weight changes (based on percent) of athymic mice following administration of 20K conjugates as part of a maximum tolerated dose study described in Example 15.

FIG. 3 is a plot of mean tumor growth volume following administration of selected 20K conjugates as part of a xenograft study further discussed in Example 16.

FIG. 4 is a plot of mean tumor growth volume following administration of selected 20K conjugates as part of a xenograft study further discussed in Example 16.

DETAILED DESCRIPTION

Various aspects of the invention now will be described more fully hereinafter. Such aspects may, however, be embodied in many different focus and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

DEFINITIONS

It must be noted that, as used in this specification, the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a “polymer” includes a single polymer as well as two or more of the same or different polymers, reference to a “conjugate” refers to a single conjugate as well as two or more of the same or different conjugates, reference to an “excipient” includes a single excipient as well as two or more of the same or different excipients, and the like.

In describing and claiming the present invention, the following terminology will be used in accordance with the definitions described below.

A “functional group” is a group that may be used, under normal conditions of organic synthesis, to form a covalent linkage between the entity to which it is attached and another entity, which typically bears a further functional group. The functional group generally includes multiple bond(s) and/or heteroatom(s). Preferred functional groups for use in the polymers of the invention are described below.

The term “reactive” refers to a functional group that reacts readily or at a practical rate under conventional conditions of organic synthesis. This is in contrast to those groups that either do not react or require strong catalysts or impractical reaction conditions in order to react (i.e., a “nonreactive” or “inert” group).

“Not readily reactive”, with reference to a functional group present on a molecule in a reaction mixture, indicates that the group remains largely intact under conditions effective to produce a desired reaction in the reaction mixture.

An “activated derivative” of a carboxylic acid refers to a carboxylic acid derivative which reacts readily with nucleophiles, generally much more readily than the underivatized carboxylic acid. Activated carboxylic acids include, for example, acid halides (such as acid chlorides), anhydrides, carbonates, and esters. Such esters include, for example, imidazolyl esters, and benzotriazole esters, and imide esters, such as N-hydroxysuccinimidyl (NHS) esters. An activated derivative may be formed in situ by reaction of a carboxylic acid with one of various reagents, e.g. benzotriazol-1-yloxy tripyrrolidinophosphonium hexafluorophosphate (PyBOP), preferably used in combination with 1-hydroxy benzotriazole (HOBT) or 1-hydroxy-7-azabenzotriazole (HOAT); O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HATU); or bis(2-oxo-3-oxazolidinyl)phosphinic chloride (BOP-Cl).

A “chemical equivalent” of a functional group is one that possesses essentially the same type of reactivity as the functional group. For instance, one functional group that undergoes an SN2 reaction is considered to be a functional equivalent of another such functional group.

A “protecting group” is a moiety that prevents or blocks reaction of a particular chemically reactive functional group in a molecule under certain reaction conditions. The protecting group will vary depending upon the type of chemically reactive group being protected as well as the reaction conditions to be employed and the presence of additional reactive or protecting groups in the molecule. Functional groups that may be protected include, by way of example, carboxylic acid groups, amino groups, hydroxyl groups, thiol groups, carbonyl groups and the like. Representative protecting groups for carboxylic acids include esters (such as a p-methoxybenzyl ester), amides and hydrazides; for amino groups, carbamates (such as tert-butoxycarbonyl) and amides; for hydroxyl groups, ethers and esters; for thiol groups, thioethers and thioesters; for carbonyl groups, acetals and ketals; and the like. Such protecting groups are well-known to those skilled in the art and are described, for example, in T. W. Greene and G. M. Wuts, Protecting Groups in Organic Synthesis, Third Edition, Wiley, New York, 1999, and in P. J. Kocienski, Protecting Groups, Third Ed., Thieme Chemistry, 2003, and references cited therein.

A functional group in “protected form” refers to a functional group bearing a protecting group. As used herein, the term “functional group” or any synonym thereof is meant to encompass protected forms thereof.

“PEG” or “poly(ethylene glycol)” as used herein, is meant to encompass any water-soluble poly(ethylene oxide). Typically, PEGs for use in the present invention will comprise one of the two following structures: “—(CH₂CH₂O)_(n)—” or “—(CH₂CH₂O)_(n-1)CH₂CH₂—,” depending upon whether or not the terminal oxygen(s) has been displaced, e.g., during a synthetic transformation, or, e.g., the identity of adjacent functional groups. The variable (n) typically ranges from 3 to about 3000, and the terminal groups and architecture of the overall PEG may vary. When PEG or a conjugate comprising a PEG segment further comprises a spacer or a linker as in Compound I above (to be described in greater detail below), the atoms comprising the spacer (X) or linker (O), when covalently attached to a PEG segment, do not result in formation of (i) an oxygen-oxygen bond (—O—O—, a peroxide linkage), or (ii) a nitrogen-oxygen bond (N—O, O—N). PEGs for use in the invention include PEGs having a variety of molecular weights, structures or geometries to be described in greater detail below.

“Water-soluble,” in the context of a polymer of the invention or a “water-soluble polymer segment” is any segment or polymer that is soluble in water at room temperature. Typically, a water-soluble polymer or segment will transmit at least about 75%, more preferably at least about 95% of light, transmitted by the same solution after filtering. On a weight basis, a water-soluble polymer or segment thereof will preferably be at least about 35% (by weight) soluble in water, more preferably at least about 50% (by weight) soluble in water, still more preferably about 70% (by weight) soluble in water, and still more preferably about 85% (by weight) soluble in water. It is most preferred, however, that the water-soluble polymer or segment is about 95% (by weight) soluble in water or completely soluble in water.

“Non-naturally occurring” with respect to a polymer means a polymer that in its entirety is not found in nature. A non-naturally occurring polymer may however contain one or more subunits or segments of subunits that are naturally occurring, so long as the overall polymer structure is not found in nature.

“Molecular mass” in the context of a water-soluble polymer such as PEG, refers to the nominal average molecular mass of a polymer, typically determined by size exclusion chromatography, light scattering techniques, or intrinsic viscosity determination in water or organic solvents. Molecular weight in the context of a water-soluble polymer, such as PEG, can be expressed as either a number-average molecular weight or a weight-average molecular weight. Unless otherwise indicated, all references to molecular weight herein refer to the number-average molecular weight. Both molecular weight determinations, number-average and weight-average, can be measured using gel permeation chromatographic techniques. Other methods for measuring molecular weight values can also be used, such as the use of end-group analysis or the measurement of colligative properties (e.g., freezing-point depression, boiling-point elevation, or osmotic pressure) to determine number-average molecular weight or the use of light scattering techniques, ultracentrifugation or viscometry to determine weight-average molecular weight. The polymers of the invention are typically polydisperse (i.e., number-average molecular weight and weight-average molecular weight of the polymers are not equal), possessing low polydispersity values such as less than about 1.2, less than about 1.15, less than about 1.10, less than about 1.05, and less than about 1.03. As used herein, references will at times be made to a single water-soluble polymer having either a weight-average molecular weight or number-average molecular weight; such references will be understood to mean that the single-water soluble polymer was obtained from a composition of water-soluble polymers having the stated molecular weight.

The term “linker” is used herein to refer to an atom or a collection of atoms used to link interconnecting moieties, such as an organic radical core and a polymer segment, POLY¹. A linker moiety may be hydrolytically stable or may include a releasable linkage (e.g., a physiologically hydrolyzable linkage, an enzymatically degradable linkage, or another linkage that cleaves in vivo).

The term “spacer” is used herein to refer to an atom or a collection of atoms used to link interconnecting moieties, such as POLY¹ and a drug. A spacer moiety may be hydrolytically stable or may include a physiologically hydrolyzable or enzymatically degradable linkage.

A “hydrolyzable” bond is a relatively weak bond that reacts with water (i.e., is hydrolyzed) under physiological conditions. The tendency of a bond to hydrolyze in water will depend not only on the general type of linkage connecting two central atoms but also on the substituents attached to these central atoms. Illustrative hydrolytically unstable linkages include carboxylate ester, phosphate ester, anhydrides, acetals, ketals, acyloxyalkyl ether, imines, orthoesters, peptides and oligonucleotides.

An “enzymatically degradable linkage” means a linkage that is subject to degradation by one or more enzymes. Such a linkage requires the action of one or more enzymes to effect degradation.

A “hydrolytically stable” linkage or bond refers to a chemical bond, typically a covalent bond, that is substantially stable in water, that is to say, does not undergo hydrolysis under physiological conditions to any appreciable extent over an extended period of time. Examples of hydrolytically stable linkages include but are not limited to the following: carbon-carbon bonds (e.g., in aliphatic chains), ethers, amides, urethanes, and the like. Generally, a hydrolytically stable linkage is one that exhibits a rate of hydrolysis of less than about 1-2% per day under physiological conditions. Hydrolysis rates of representative chemical bonds can be found in most standard chemistry textbooks.

“Multi-armed” in reference to the geometry or overall structure of a polymer refers to polymer having 3 or more polymer-containing “arms” connected to a “core” molecule or structure. Thus, a multi-armed polymer may possess 3 polymer arms, 4 polymer aims, 5 polymer arms, 6 polymer arms, 7 polymer arms, 8 polymer arms or more, depending upon its configuration and core structure. One particular type of highly branched polymer is a dendritic polymer or dendrimer, that, for the purposes of the invention, is considered to possess a structure distinct from that of a multi-armed polymer. That is to say, a multi-armed polymer as referred to herein explicitly excludes dendrimers. Additionally, a multi-armed polymer as provided herein possesses a non-crosslinked core.

A “dendrimer” is a globular, size monodisperse polymer in which all bonds emerge radially from a central focal point or core with a regular branching pattern and with repeat units that each contribute a branch point. Dendrimers are typically formed using a nano-scale, multistep fabrication process. Each step results in a new “generation” that has two or more times the complexity of the previous generation. Dendrimers exhibit certain dendritic state properties such as core encapsulation, making them unique from other types of polymers.

“Branch point” refers to a bifurcation point comprising one or more atoms at which a polymer splits or branches from a linear structure into one or more additional polymer arms. A multi-arm polymer may have one branch point or multiple branch points, so long as the branches are not regular repeats resulting in a dendrimer.

“Substantially” or “essentially” means nearly totally or completely, for instance, 95% or greater of some given quantity.

“Alkyl” refers to a hydrocarbon chain, typically ranging from about 1 to 20 atoms in length. Such hydrocarbon chains are preferably but not necessarily saturated and may be branched or straight chain, although typically straight chain is preferred. Exemplary alkyl groups include methyl, ethyl, isopropyl, n-butyl, n-pentyl, 2-methyl-1-butyl, 3-pentyl, 3-methyl-3-pentyl, and the like. As used herein, “alkyl” includes cycloalkyl when three or more carbon atoms are referenced.

“Lower alkyl” refers to an alkyl group containing from 1 to 6 carbon atoms, and may be straight chain or branched, as exemplified by methyl, ethyl, n-butyl, i-butyl, t-butyl.

“Cycloalkyl” refers to a saturated or unsaturated cyclic hydrocarbon chain, including bridged, fused, or Spiro cyclic compounds, preferably made up of 3 to about 12 carbon atoms, more preferably 3 to about 8.

“Non-interfering substituents” are those groups that, when present in a molecule, are typically non-reactive with other functional groups contained within the molecule.

The term “substituted” as in, for example, “substituted alkyl,” refers to a moiety (e.g., an alkyl group) substituted with one or more non-interfering substituents, such as, but not limited to: C₃-C₈ cycloalkyl, e.g., cyclopropyl, cyclobutyl, and the like; halo, e.g., fluoro, chloro, bromo, and iodo; cyano; alkoxy, lower phenyl; substituted phenyl; and the like. For substitutions on a phenyl ring, the substituents may be in any orientation (i.e., ortho, meta, or para).

“Alkoxy” refers to an —O—R group, wherein R is alkyl or substituted alkyl, preferably C₁-C₂₀ alkyl (e.g., methoxy, ethoxy, propyloxy, etc.), preferably C₁-C₇.

As used herein, “alkenyl” refers to a branched or unbranched hydrocarbon group of 1 to 15 atoms in length, containing at least one double bond, such as ethenyl (vinyl), 2-propen-1-yl (allyl), isopropenyl, 3-buten-1-yl, and the like.

The term “alkynyl” as used herein refers to a branched or unbranched hydrocarbon group of 2 to 15 atoms in length, containing at least one triple bond, ethynyl, 1-propynyl, 3-butyn-1-yl, 1-octyn-1-yl, and so forth.

“Aryl” means one or more aromatic rings, each of 5 or 6 core carbon atoms. Aryl includes multiple aryl rings that may be fused, as in naphthyl or unfused, as in biphenyl. Aryl rings may also be fused or unfused with one or more cyclic hydrocarbon, heteroaryl, or heterocyclic rings. As used herein, “aryl” includes heteroaryl.

“Heteroaryl” is an aryl group containing from one to four heteroatoms, preferably N, O, or S, or a combination thereof. Heteroaryl rings may also be fused with one or more cyclic hydrocarbon, heterocyclic, aryl, or heteroaryl rings.

“Heterocycle” or “heterocyclic” means one or more rings of 5-12 atoms, preferably 5-7 atoms, with or without unsaturation or aromatic character and having at least one ring atom which is not a carbon. Preferred heteroatoms include sulfur, oxygen, and nitrogen.

“Substituted heteroaryl” is heteroaryl having one or more non-interfering groups as substituents.

“Substituted heterocycle” is a heterocycle having one or more side chains formed from non-interfering substituents.

“Electrophile” refers to an ion, atom, or collection of atoms that may be ionic, having an electrophilic center, i.e., a center that is electron seeking, capable of reacting with a nucleophile.

“Nucleophile” refers to an ion or atom or collection of atoms that may be ionic, having a nucleophilic center, i.e., a center that is seeking an electrophilic center, and capable of reacting with an electrophile.

“Active agent” as used herein includes any agent, drug, compound, and the like which provides some pharmacologic, often beneficial, effect that can be demonstrated in vivo or in vitro. As used herein, these terms further include any physiologically or pharmacologically active substance that produces a localized or systemic effect in a patient.

“Pharmaceutically acceptable excipient” or “pharmaceutically acceptable carrier” refers to an excipient that can be included in the compositions of the invention and that causes no significant adverse toxicological effects to the patient.

“Pharmacologically effective amount,” “physiologically effective amount,” and “therapeutically effective amount” are used interchangeably herein to mean the amount of an active agent present in a pharmaceutical preparation that is needed to provide a desired level of active agent and/or conjugate in the bloodstream or in a target tissue or site in the body. The precise amount will depend upon numerous factors, e.g., the particular active agent, the components and physical characteristics of pharmaceutical preparation, intended patient population, patient considerations, and the like, and can readily be determined by one skilled in the art, based upon the information provided herein and available in the relevant literature.

“Multi-functional” in the context of a polymer of the invention means a polymer having 3 or more functional groups, where the functional groups may be the same or different, and are typically present on the polymer termini. Multi-functional polymers of the invention will typically contain from about 3-100 functional groups, or from 3-50 functional groups, or from 3-25 functional groups, or from 3-15 functional groups, or from 3 to 10 functional groups, i.e., contains 3, 4, 5, 6, 7, 8, 9 or 10 functional groups. Typically, in reference to a polymer precursor used to prepare a polymer conjugate of the invention, the polymer possesses 3 or more polymer arms having at the terminus of each arm a functional group suitable for coupling to an active agent moiety via a hydrolyzable ester linkage. Typically, such functional groups are the same.

A basic or acidic reactant described herein includes neutral, charged, and any corresponding salt forms thereof.

“Polyolefinic alcohol” refers to a polymer comprising an olefin polymer backbone, such as polyethylene, having multiple pendant hydroxyl groups attached to the polymer backbone. An exemplary polyolefinic alcohol is polyvinyl alcohol.

As used herein, “non-peptidic” refers to a polymer backbone substantially free of peptide linkages. However, the polymer may include a minor number of peptide linkages spaced along the repeat monomer subunits, such as, for example, no more than about 1 peptide linkage per about 50 monomer units.

The terms “subject,” “individual” or “patient” are used interchangeably herein and refer to a vertebrate, preferably a mammal. Mammals include, but are not limited to, murines, rodents, simians, humans, farm animals, sport animals and pets. Such subjects are typically suffering from or prone to a condition that can be prevented or treated by administration of a polymer of the invention, typically but not necessarily in the form of a polymer-active agent conjugate as described herein.

“Treatment” or “treating” of a particular condition includes: (1) preventing such a condition, i.e., causing the condition not to develop, or to occur with less intensity or to a lesser degree in a subject that may be exposed to or predisposed to the condition but does not yet experience or display the condition; and (2) inhibiting the condition, i.e., arresting the development or reversing the condition.

“Optional” or “optionally” means that the subsequently described circumstance may or may not occur, so that the description includes instances where the circumstance occurs and instances where it does not.

A “residue” refers to a portion of compound remaining or present following a chemical reaction (whether a synthetic chemical reaction or following compound releasing-chemical reaction). For example, a polyol that is used to form a multi-arm polymer will have a “residue” of that polyol present in the multi-aim polymer.

A “polyol” is an alcohol containing more than two hydroxyl groups, where the prefix “poly” in this instance refers to a plurality of a certain feature rather than to a polymeric structure. Similarly, a polythiol is a thiol containing more than two thiol (—SH) groups, and a polyamine is an amine containing more than two amino groups.

As previously indicated, one or more embodiments of the invention relate to conjugates having a structure encompassed by Formula I. The conjugates of the invention are both prodrugs (given the presence of a spacer moiety that includes a releasable linkage) and “multi-armed.” Thus, upon administration to an individual, the prodrug releases in vivo a compound lacking attachment to the water-soluble, non-peptidic polymer via in vivo cleavage. For example, in vivo cleavage of an ester may occur with or without the benefit of an esterase. In an additional example, in vivo cleavage of an amide may occur with the benefit of, for example, a peptidase (such as a γ-glutamyl hydrolase). Because the conjugates are multi-armed, release occurs multiple times, thereby delivering following administration several moles of released compound for each mole of starting conjugate.

Thus, exemplary conjugates of the invention have a structure encompassed by the formula:

wherein:

R is a residue of a polyol, polythiol or polyamine bearing at from 3 to about 50 hydroxyl, thiol or amino groups;

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each Q is a linker (and, in one or more embodiments, a hydrolytically stable linker);

each POLY¹ is a water-soluble, non-peptidic polymer;

each X is spacer moiety that optionally includes a releasable linkage (e.g., a hydrolyzable linkage, an enzymatically degradable linkage, and so forth); and

q is a positive integer from 3 to about 50 (e.g., 4),

and pharmaceutically acceptable salts and solvates thereof.

As contemplated by the above structure, the conjugate has “q” number of arms, i.e., from 3 to about 50. An exemplary number of arms includes 3, 4, 5, 6, 7, 9, and 10. In one or more embodiments, the conjugates of the invention are prepared from multi-armed polymer reagents, which, in turn, are prepared from multi-arm polymers based on a multi-arm core molecule.

For example, in one approach, a multi-arm polymer can be prepared from a multi-arm core molecule by effectively “growing” a polymer onto each terminus of a multi-arm core molecule. By way of non-limiting example, it is possible to synthesize a polymer arm onto a polyol (e.g., pentaerythritol, diglycerol, etc.) via an ethoxylation reaction. In another exemplary approach, a multi-arm polymer can be prepared from a multi-arm core molecule by attaching a water-soluble, non-peptidic polymer onto each terminus of a multi-arm core molecule. The principles of both approaches are described in the literature and in, for example, U.S. Pat. No. 7,026,440. The invention, however, is not limited with regard to the specific approach taken, so long as the conjugate is encompassed by one or more of the structures provided herein.

The Residue of the Polyol, Polythiol, or Polyamine, “R”

In one or more embodiments, the residue of the polyol, polythiol or polyamine, “R,” is an organic radical-containing moiety. The polyol, polythiol or polymamine from which “R” is derived possesses from about 3 to about 150 carbon atoms (e.g., from about 3 to about 50 carbon atoms, such as 3, 4, 5, 6, 7, 8, 9, and 10). The residue may contain one more heteroatoms (e.g., O, S, or N). In addition, the residue may be linear. In some instances, the residue may be cyclic.

As previously indicated, the residue of the polyol, polythiol or polyamine, “R,” that forms the basis of the branching for the multi-armed conjugates provided herein, originated from a corresponding polyol, polythiol or polyamine (prior to be incorporated into the multi-arm structures containing a water-soluble, non-peptidic polymer). In one or more embodiments, the corresponding polyol, polythiol, or a polyamine bears at least three hydroxyl, thiol, or amino groups, respectively, available for polymer attachment. A “polyol” is a molecule comprising three or more hydroxyl groups. A “polythiol” is a molecule that comprises three or more thiol groups. A “polyamine” is a molecule comprising three or more amino groups.

In one or more embodiments, the polyol, polyamine or polythiol will typically contain 3 to about 25 hydroxyl, or amino groups or thiol groups, respectively, such as from 3 to about 10 (i.e., 3, 4, 5, 6, 7, 8, 9, 10) hydroxyl, amino groups or thiol groups, respectively, preferably from 3 to about 8 (i.e., 3, 4, 5, 6, 7, or 8) hydroxyl, amino groups or thiol groups, respectively. In one or more embodiments, the number of atoms between each hydroxyl, thiol, or amino group will vary, although lengths of from about 1 to about 20 (e.g., from 1 to about 5) intervening atoms, such as carbon atoms, between each hydroxyl, thiol or amino group, are exemplary. In referring to intervening core atoms and lengths, —CH₂— is considered as having a length of one intervening atom, —CH₂CH₂— is considered as having a length of two atoms, and so forth.

Exemplary polyols and polyamines (for which corresponding residues could be present in the conjugates provided herein) have a (Radical)-(OH)_(q) and (Radical)-(NH₂)_(q) structures, respectively, where (Radical) corresponds to an organic-containing radical and q is a positive integer from 3 to about 50. Note that in Formula I, the variable “Q,” when taken together with R, typically represents a residue of the core organic radical as described herein. That is to say, when describing polyols, polythiols and polymer amines, particularly by name, these molecules are being referenced in their form prior to incorporation into a water-soluble polymer-containing structure. So, for example, a conjugate of Formula I wherein R is a residue of the polyol, pentaerythritol [C(CH₂OH)₄], the residue “R” includes carbon (i.e., “C,”) and, together with “Q,” represents “C(CH₂O—)₄.”

Illustrative polyols include aliphatic polyols having from 1 to 10 carbon atoms and from 3 to 10 hydroxyl groups, including for example, trihydroxyalkanes, tetrahydroxyalkanes, polyhydroxy alkyl ethers, polyhydroxyalkyl polyethers, and the like. Cycloaliphatic polyols include straight chained or closed-ring sugars and sugar alcohols, such as mannitol, sorbitol, inositol, xylitol, quebrachitol, threitol, arabitol, erythritol, adonitol, dulcitol, facose, ribose, arabinose, xylose, lyxose, rhamnose, galactose, glucose, fructose, sorbose, mannose, pyranose, altrose, talose, tagitose, pyranosides, sucrose, lactose, maltose, and the like. Additional examples of aliphatic polyols include derivatives of glucose, ribose, mannose, galactose, and related stereoisomers. Aromatic polyols may also be used, such as 1,1,1-tris(4′-hydroxyphenyl)alkanes, such as 1,1,1-tris(4-hydroxyphenyl)ethane, 2,6-bis(hydroxyalkyl)cresols, and the like. Other core polyols that may be used include polyhydroxycrown ethers, cyclodextrins, dextrins and other carbohydrates (e.g., monosaccharides, oligosaccharides, and polysaccharides, starches and amylase).

Exemplary polyols include glycerol, trimethylolpropane, pentaerythritol, dipentaerythritol, tripentaerythritol, ethoxylated forms of glycerol, trimethylolpropane, pentaerythritol, dipentaerythritol, tripentaerythritol. Also, preferred are reducing sugars such as sorbitol and glycerol oligomers, such as diglycerol, triglycerol, hexaglycerol and the like. A 21-arm polymer can be synthesized using hydroxypropyl-β-cyclodextrin, which has 21 available hydroxyl groups. Additionally, a polyglycerol having an average of 24 hydroxyl groups is also included as an exemplary polyol.

Exemplary polyamines include aliphatic polyamines such as diethylene triamine, N,N,N″-trimethyldiethylene triamine, pentamethyl diethylene triamine, triethylene tetramine, tetraethylene pentamine, pentaethylene hexamine, dipropylene triamine, tripropylene tetramine, bis-(3-aminopropyl)-amine, bis-(3-aminopropyl)-methylamine, and N,N-dimethyl-dipropylene-triamine. Naturally occurring polyamines that can be used in the present invention include putrescine, spermidine, and spermine. Numerous suitable pentamines, tetramines, oligoamines, and pentamidine analogs suitable for use in the present invention are described in Bacchi et al. (2002) Antimicrobial Agents and Chemotherapy, 46(1):55-61, which is incorporated by reference herein.

Provided below are illustrative structures corresponding to residues of polyols [although each structure is depicted with the oxygen atom (“O”) derived from the corresponding hydroxyl group, each “O” can be substituted with sulfur (“S”) or NH to depict the corresponding residue of a polythiol or polyamine, respectively). Note that the residues shown below would be understood in terms of compounds of Formula I as corresponding to “R” and “Q.” In any event, conjugates based on any of the illustrative structures set forth below are included as part of the invention.

wherein m is a positive integer from 0-40 [preferably O-10, e.g., 0-5 (i.e., 0, 1, 2, 3, 4, 5)].

Water-soluble, non-peptidic-containing multi-arm polymers (used as, for example, multi-arm polymeric reagents to prepare conjugates encompassed by Formula) based on the above-described polyols, polythiols and polyamines and others are described in WO 2007/098466, WO 2010/019233 and U.S. Pat. No. 7,744,861. These references and others describe methods for preparing such multi-arm polymers. In addition, some multi-arm polymers are available commercially from, for example, Creative PEGWorks (Winston Salem, N.C. USA), SunBio PEG-Shop (SunBio USA, Orinda, Calif.), JenKem Technology USA (Allen, Tex.), and NOF America Corporation (White Plains, N.Y.).

The Linker, “Q”

The linker, Q, serves to connect the residue of the polyol, polythiol or polyamine bearing at from 3 to about 50 hydroxyl, thiol or amino groups, “R,” to each water-soluble, non-peptidic polymer, POLY¹, in conjugates according to Formula I. In this regard, the invention is not limited with respect to the specific linker used. In one or more embodiments, the linker between the residue, “R,” and the water-soluble, non-peptidic polymer, POLY¹, is a hydrolytically stable linker).

In one or more embodiments of the invention, the linker, Q, is influenced by the approach used to form the multi-arm polymer employed in preparing the conjugates of the invention. For example, if a water-soluble, non-peptidic polymer bearing a functional group reactive to a hydroxyl, thiol or amine is reacted with a polyol, polythiol or polyamine, respectively, the linker, Q, may include one or more atoms incorporating the bond formed between the termini of the polyol, polythiol or polamine and the beginning of the repeating monomers of the water-soluble, non-peptidic polymer, POLY¹. Illustrative linking chemistries in this regard (along with the resulting linkers) are described in the literature and in, for example, Wong (1991) “Chemistry of Protein Conjugation and Crosslinking”, CRC Press, Boca Raton, Fla., and Brinkley (1992) Bioconjug. Chem. 3:2013.

In one or more embodiments of conjugates of Formula I, Q contains at least one heteratom such as O, or S, or NH, where the atom proximal to R in Q, when taken together with R, typically represents a residue of an organic radical-containing core of the polyol, polythiol or polyamine. Generally, the linker, Q, contains from 1 to about 10 atoms (e.g., from 1 to about 5 atoms). The linker, Q, typically contains a number of atoms selected from the group consisting of: 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10. Illustrative Qs include O, S, —NH—, —NH—C(O)— and —C(O)—NH—.

The Water-soluble, Non-peptidic Polymer, POLY¹

The conjugates of the invention include several water-soluble, non-peptidic polymers as part of the overall structure. With respect to conjugates, each the water-soluble, non-peptidic polymer in the conjugate (e.g., POLY¹ in connection with compounds encompassed by Formula I) is independently selected, although preferably, each water-soluble, non-peptidic polymer is the same polymer type. That is, for example, each POLY¹ in the multi-armed conjugate is the same.

Any of a variety of water-soluble, non-peptidic polymers that are non-peptidic and water-soluble can be used in the multi-arm conjugates and the invention is not limited in this regard. Examples water-soluble, non-peptidic polymers include poly(alkylene glycols), copolymers of ethylene glycol and propylene glycol, poly(olefinic alcohol), poly(vinylpyrrolidone), poly(hydroxyalkylmethacrylamide), poly(hydroxyalkylmethacrylate), poly(saccharides), poly(α-hydroxy acid), poly(acrylic acid), poly(vinyl alcohol), polyphosphazene, polyoxazoline, poly(N-acryloylmorpholine), such as described in U.S. Pat. No. 5,629,384, and copolymers, terpolymers, and mixtures of any one or more of the above.

When the water-soluble, non-peptidic polymer, e.g., POLY¹, is PEG, its structure typically comprises —(CH₂CH₂O)_(n)— (wherein the terminal ethylene is covalently attached to “Q” and the terminal oxygen is attached to “X,” with respect to conjugates encompassed by Formula I), where n may range from about 5 to about 400, preferably from about 10 to about 350, or from about 20 to about 300.

Exemplary molecular weights for the water-soluble, non-peptidic polymer (e.g., POLY¹) include: about 200, about 250, about 300, about 400, about 500, about 600, about 700, about 800, about 900, about 1,000, about 1,500, about 2,000, about 3,000, about 4,000, about 5,000, about 6,000, about 7,000, about 7,500, about 8,000, about 9,000, about 10,000, about 12,000, about 15,000, about 17,500, about 18,000, about 19,000 and about 20,000 Daltons. In terms of the molecular weight of the multi-armed polymer, exemplary molecular weights include: about 800, about 1,000, about 1,200, about 1,600, about 2,000, about 2,400, about 2,800, about 3,200, about 3,600, about 4,000, about 5,000, about 6,000, about 8,000, about 10,000, about 12,000, about 15,000, about 16,000, about 20,000, about 24,000, about 25,000, about 28,000, about 30,000, about 32,000, about 36,000, about 40,000, about 45,000, about 48,000, about 50,000, about 60,000, about 80,000 and about 100,000 Datltons. With respect to molecular weight ranges for the multi-armed polymer, exemplary ranges include: from about 800 to about 80,000 Daltons; from about 900 to about 70,000 Daltons; From about 1,000 to about 40,000 Daltons; from 5,000 to about 30,000 Daltons; and from about 20,000 to about 80,000 Daltons.

The Spacer Moiety, X

The spacer moiety, X, serves to connect the water-soluble, non-peptidic polymer (e.g., POLY¹ in conjugates according to Formula I) to the cabazitaxel-based compound. Optionally included as part of the spacer moiety is a releasable linkage (such as a hydrolyzable linkage or an enzymatically degradable linkage). In this regard, the invention is not limited with respect to the specific linker used.

As part of the spacer moiety, X, the portions of the spacer moiety may include one or more components selected from the group consisting of —O—, —S—, —NH—, —C(O)—, —O—C(O)—, —C(O)—O—, —C(O)—NH—, —NH—C(O)—NH—, —O—C(O)—NH—, —C(S)—, —CH₂—, —CH₂—CH₂—, —CH₂—CH₂—CH₂—, —CH₂—CH₂—CH₂—CH₂—, —O—CH₂—, —CH₂—O—, —O—CH₂—CH₂—, —CH₂—O—CH₂—, —CH₂—CH₂—O—, —O—CH₂—CH₂—CH₂—, —CH₂—O—CH₂—CH₂—, —CH₂—CH₂—O—CH₂—, —CH₂—CH₂—CH₂—O—, —O—CH₂—CH₂—CH₂—CH₂—, —CH₂—O—CH₂—CH₂—CH₂—, —CH₂—CH₂—O—CH₂—CH₂—, —CH₂—CH₂—CH₂—O—CH₂—, —CH₂—CH₂—CH₂—CH₂—O—, —C(O)—NH—CH₂—, —C(O)—NH—CH₂—CH₂—, —CH₂—C(O)—NH—CH₂—, —CH₂—CH₂—C(O)—NH—, —C(O)—NH—CH₂—CH₂—CH₂—, —CH₂—C(O)—NH—CH₂—CH₂—, —CH₂—CH₂—C(O)—NH—CH₂—, —CH₂—CH₂—CH₂—C(O)—NH—, —C(O)—NH—CH₂—CH₂—CH₂—CH₂—, —CH₂—C(O)—NH—CH₂—CH₂—CH₂—, —CH₂—CH₂—C(O)—NH—CH₂—CH₂—, —CH₂—CH₂—CH₂—C(O)—NH—CH₂—, —CH₂—CH₂—CH₂—C(O)—NH—CH₂—CH₂—, —CH₂—CH₂—CH₂—CH₂—C(O)—NH—, —C(O)—O—CH₂—, —CH₂—C(O)—O—CH₂—, —CH₂—CH₂—C(O)—O—CH₂—, —C(O)—O—CH₂—CH₂—, —NH—C(O)—CH₂—, —CH₂—NH—C(O)—CH₂—, —CH₂—CH₂—NH—C(O)—CH₂—, —NH—C(O)—CH₂—CH₂—, —CH₂—NH—C(O)—CH₂—CH₂—, —CH₂—CH₂—NH—C(O)—CH₂—CH₂—, —C(O)—NH—CH₂—, —C(O)—NH—CH₂—CH₂—, —O—C(O)—NH—CH₂—, —O—C(O)—NH—CH₂—CH₂—, —O—C(O)—NH—CH₂—CH₂—CH₂—, —NH—CH₂—, —NH—CH₂—CH₂—, —CH₂—NH—CH₂—, —CH₂—CH₂—NH—CH₂—, —C(O)—CH₂—, —C(O)—CH₂—CH₂—, —CH₂—C(O)—CH₂—, —CH₂—CH₂—C(O)—CH₂—, —CH₂—CH₂—C(O)—CH₂—CH₂—, —CH₂—CH₂—C(O)—, —CH₂—CH₂—CH₂—C(O)—NH—CH₂—CH₂—NH—, —CH₂—CH₂—CH₂—C(O)—NH—CH₂—CH₂—NH—C(O)—, —CH₂—CH₂—CH₂—C(O)—NH—CH₂—CH₂—NH—C(O)—CH₂—, —CH₂—CH₂—CH₂—C(O)—NH—CH₂—CH₂—NH—C(O)—CH₂—CH₂—, —(CH₂)₀₋₆C(O)—, —C(O)—(CH₂)₀₋₆—, —O—C(O)—NH—[CH₂]₀₋₆—(OCH₂CH₂)₀₋₂—, —C(O)—NH—(CH₂)₁₋₆—NH—C(O)—, —[NH(CHR⁶)₁₋₆C(O)]₁₋₄— (and wherein each R⁶ is independently selected from the group consisting of H, lower alkyl and arylalkyl), —NH(CH₂CH₂O)₁₋₁₀ (CH₂)₁₋₆—, —NH(CH₂CH₂O)₁₋₁₀(CH₂)₁₋₅(CHCH₃)(CH₂)₀₋₄—, and —NH—C(O)—NH—(CH₂)₁₋₆—NH—C(O)—. For purposes of the present disclosure, however, a series of atoms is not a spacer moiety when the series of atoms is immediately adjacent to a water-soluble polymer and the series of atoms is but another monomer, such that the proposed spacer moiety would represent a mere extension of the polymer chain. X can also be L₁-L₂, wherein each of L₁ and L₂ is independently an “X” as defined herein.

In one or more embodiments of the invention, the spacer moiety, X, may include a cycloalkylene group, e.g. 1,3- or 1,4-cyclohexylene.

In one or more embodiments of the invention, the spacer moiety, X, has an atom length of from about 1 atom to about 50 atoms, or more preferably from about 1 atom to about 25 atoms, or even more preferably from about 1 atom to about 10 atoms. Typically, the spacer moiety is of an atom length selected from the group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10. When considering atom chain length, only atoms contributing to the overall distance are considered. For example, a spacer having the structure, —CH₂—C(O)—NH—CH₂ CH₂ O—CH₂ CH₂ O—C(O)—C— has a chain length of 11 atoms, since substituents are not considered to contribute to the length of the spacer.

In one or more embodiments of the invention, the spacer moiety, X, possesses the structure: Y—Z, where Y is a spacer fragment covalently attached to Z, a hydrolytically or enzymatically degradable linkage. In certain embodiments, Z itself may not constitute a hydrolytically or enzymatically degradable linkage, however, when taken together with Y, or at least a portion of Y, forms a linkage that is hydrolytically or enzymatically degradable. Also, Z taken together with the —C(O)—O— to which it is attached, is potentially a hydrolytically or enzymatically degradable linkage. Preferred enzymatically degradable groups include esters, disulfides, dipeptides, tripeptides, and tetrapeptides. Also preferred are certain amides such as glutamides, peptoids and peptide mimics.

In one or more embodiments of the invention, when the spacer moiety, X, is Y—Z, Y will have the structure: —(CR_(x)R_(y))_(a)—K—(CR_(x)R_(y))_(b)—(CH₂CH₂O)_(n)—, wherein each R_(x) and R_(y), in each occurrence, is independently H or an organic radical selected from the group consisting of alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, and substituted aryl, a ranges from 0 to 12 (i.e., can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12), b ranges from 0 to 12 (i.e., can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12), K is selected from —C(O)—, —C(O)NH—, —NH—C(O)—, —O—, —S—, O—C(O)—, C(O)—O—, —O—C(O)—O—, O—C(O)—NH—, —NH—C(O)—O—, and c ranges from 0 to 25, and Z is selected from C(O)—O—, O—C(O)—O—, —O—C(O)—NH—, and —NH—C(O)—O—. The particular structure of K and of Z will depend upon the values of each of a, b, and c, such that none of the following linkages result in the overall structure of the spacer moiety, X: —O—O—, NH—O—, and —NH—NH—.

In one or more embodiments of the invention, when the spacer moiety, X, is Y—Z, Y will have the structure: —(CR_(x)R_(y))_(a)—K—(CR_(x)R_(y))_(b)—(CH₂CH₂NH)_(c)—, where the variables R_(x), R_(y), a, b and c have the values described in the previous paragraph.

In one or more embodiments of the invention, R_(x) and R_(y) (as set forth in each of the two preceding paragraphs) is, in each occurrence, independently H or lower alkyl. In one or more embodiments of the invention, R_(x) and R_(y) are, in each occurrence, H. In yet another embodiment, “a” ranges from 0 to 5, i.e., is selected from 0, 1, 2, 3, 4, or 5. In yet another embodiment, b ranges from 0 to 5, i.e., is selected from 0, 1, 2, 3, 4, or 5. In yet another embodiment, c ranges from 0 to 10. In yet another embodiment, K is —C(O)—NH.

In one or more embodiments, the spacer moiety, X, can also include one or more amino acid residues. In such embodiments, exemplary amino acid residues are residues from the amino acids selected from the group consisting of: alanine, valine, leucine, isoleucine, glycine, threonine, serine, cysteine, methionine, tyrosine, phenylalanine, tryptophan, aspartic acid, glutamic acid, lysine, arginine, histidine, proline, and non-naturally occurring amino acids.

The Cabazitaxel-Based Compound

The multi-arm polymer conjugates described herein include a reside of a cabazitaxel-based compound having the following structure:

wherein:

R¹ is H or methyl;

R² is H or methyl, with the provisio that R¹ and R² are not both H; and

G is H or C(O)—(CH₂)₁₋₆—NH₂.

Certain exemplary forms of the cabazitaxel-based compounds (include their synthesis) are described in U.S. Pat. Nos. 5,847,170 and 5,962,705. Other forms of the cabazitaxel-based compounds of Formula H can be prepared by one of ordinary skill in the by reference to the disclosure provided herein in view of U.S. Pat. Nos. 5,847,170 and 5,962,705 and other literature.

Compound A (the chemical formula of which is provided below) can serve as a cabazitaxel-based compound for inclusion in the polymer-containing compounds described herein.

Compound A can be prepared from 10-deacetylbaccatin III, which is a commercially available compound from Sigma-Aldrich (St. Louis, Mo.). In one exemplary approach, one of any of several synthetic methods is used to protect the “10 position” hydroxyl. One such approach is schematically provided below (where the “10 position” is protected with a triethylsilyl protecting group):

Thereafter, the 10-protected 10-deacetylbaccatin III compound can be alkylated at the 7-position with a methylating reagent (protection of the “15 position” hydroxy is not necessary). One exemplary approach for alkylating the 7-position is schematically shown below:

Thereafter, the “10 protected” form of 7-methoxy, 10-deacetylbaccatin III can be esterified followed by deprotection to form Compound A. An example the esterification and deprotection steps is depicted in the schematic below:

Compound B (the chemical formula of which is provided below) can serve as a cabazitaxel-based compound for inclusion in the polymer-containing compounds described herein.

Compound B can also be prepared from 10-deacetylbaccatin III. In one exemplary approach, one of any of several synthetic methods is used to protect the “7 position” hydroxyl. One such approach is schematically provided below (where the “7 position” is protected with a triethylsilyl protecting group):

Thereafter, the 7-protected 10-deacetylbaccatin III compound can be alkylated at the 10-position with a methylating reagent (protection of the “15 position” hydroxy is not necessary). One exemplary approach for alkylating the 10-position is schematically shown below:

Thereafter, the “7 protected” form of 10-methoxy, 10-deacetylbaccatin III can be esterified followed by deprotection to form Compound B. An example the esterification and deprotection steps is depicted in the schematic below:

Method of Preparing the Conjugates of the Invention

The conjugates of the invention can be prepared using conventional synthetic approaches of organic chemistry and the invention is not limited with respect to the manner in which the conjugates are made.

In one approach for preparing conjugates of the invention, a multi-arm polymer reagent (which can be obtained from commercially available sources, such as Creative PEGWorks, SunBio PEG-Shop, JenKem Technology USA, and NOF America Corporation, or prepared in accordance with descriptions provided in the literature) is contacted, under conjugation conditions, with an excess (typically at least a molar excess of the number of “q” polymer arms of the reagent) of the cabazitaxel-based compound of Formula II. Conjugation conditions are those conditions of temperature, pH, time, solvent, and so forth that allow for covalent attachment between a reactive group of the reagent to a functional group of the taxane-based compound. Exemplary conjugation conditions between a given polymer reagent bearing a reactive group and a corresponding functional group of a taxane-based compound will be known to one of ordinary skill in the art in view of the disclosure provided herein. See, for example, Poly(ethylene glycol) Chemistry and Biological Applications, American Chemical Society, Washington, D.C. (1997).

A multi-armed polymer reagent suitable for use in connection with conjugation conditions will typically have reactive groups selected from the group consisting of: N-succinimidyl carbonate (see e.g., U.S. Pat. Nos. 5,281,698, 5,468,478), amine (see, e.g., Buckmann et al. Makromol. Chem. 182:1379 (1981), Zalipsky et al. Eur. Polym. J. 19:1177 (1983)), hydrazide (See, e.g., Andresz et al. Makromol. Chem. 179:301 (1978)), succinimidyl propionate and succinimidyl butanoate (see, e.g., Olson et al. in Poly(ethylene glycol) Chemistry & Biological Applications, pp 170-181, Harris & Zalipsky Eds., ACS, Washington, D.C., 1997; see also U.S. Pat. No. 5,672,662), succinimidyl succinate (See, e.g., Abuchowski et al. Cancer Biochem. Biophys. 7:175 (1984) and Joppich et al., Makromol. Chem. 180:1381 (1979), succinimidyl ester (see, e.g., U.S. Pat. No. 4,670,417), benzotriazole carbonate (see, e.g., U.S. Pat. No. 5,650,234), glycidyl ether (see, e.g., Pitha et al, Eur. J. Biochem. 94:11 (1979), Elling et al., Biotech. Appl. Biochem. 13:354 (1991), oxycarbonylimidazole (see, e.g., Beauchamp, et al., Anal. Biochem. 131:25 (1983), Tondelli et al. J. Controlled Release 1:251 (1985)), p-nitrophenyl carbonate (see, e.g., Veronese, et al., Appl. Biochem. Biotech., 11:141 (1985); and Sartore et al., Appl. Biochem, Biotech., 27:45 (1991)), aldehyde (see, e.g., Harris et al. J. Polym. Sci. Chem. Ed. 22:341 (1984), U.S. Pat. No. 5,824,784, U.S. Pat. No. 5,252,714), maleimide (see, e.g., Goodson et al. Bio/Technology 8:343 (1990), Romani et al. in Chemistry of Peptides and Proteins 2:29 (1984)), and Kogan, Synthetic Comm. 22:2417 (1992)), orthopyridyl-disulfide (see, e.g., Woghiren, et al. Bioconj. Chem. 4:314 (1993)), acrylol (see, e.g., Sawhney et al., Macromolecules, 26:581 (1993)), vinylsulfone (see, e.g., U.S. Pat. No. 5,900,461). As provided in these references, exemplary conjugation conditions (including conditions of temperature, pH, time and solvent) for a given reactive group of a polymer reagent are disclosed.

In one or more embodiments, the taxane-based compound described herein may include one or more protected hydroxy groups. With respect to preparing the conjugates, in one approach, the protecting group(s) can be removed prior to conjugating the taxane-based compound with a polymer reagent. In another approach, the protecting group(s) can be removed after conjugating the taxane-based compound with a polymer reagent.

With before of after conjugation, hydroxy protecting groups can be removed by any suitable method and the invention is not limited in this regard. Exemplary approaches for removing hydroxy protecting groups include treatment with an acid (e.g., for acetyl, benzyl, β-methoxyethoxymethyl ether, dimethoxytrityl, methoxymethyl ether, methoxytrityl, p-methoxybenzyl ether, methylthiomethyl ether, pivaloyl, tetrahydropyranyl, trityl, silyl ether, and ethoxyethyl ether hydroxy protecting groups), treatment with a base (e.g., for acetyl, benzoyl and pivaloyl hydroxy protecting groups), hydrogenolysis (e.g., for benzyl, methoxytrityl, p-methoxybenzyl ether and trityl hydroxy protecting groups), oxidation (e.g., for p-methoxybenzyl ether hydroxy protecting group), and treatment with BBr₃ is methylene chloride (e.g., for methyl ether hydroxy protecting group).

Following the initial conjugation, compositions containing the conjugates of Formula I can be purified. Methods of purification and isolation include precipitation followed by filtration and drying, as well as chromatography. Suitable chromatographic methods include gel filtration chromatography, ion exchange chromatography, and flash chromatography.

Salts of the Conjugates

The conjugates may be used in their base form. In addition, the conjugates may be used in the form corresponding to a pharmaceutically acceptable salt of the conjugate, and any reference to the conjugates of the invention herein is intended to include pharmaceutically acceptable salts. If used, a salt of a compound as described herein should be both pharmacologically and pharmaceutically acceptable, but non-pharmaceutically acceptable salts may conveniently be used to prepare the free active compound or pharmaceutically acceptable salts thereof and are not excluded from the scope of this invention. Such pharmacologically and pharmaceutically acceptable salts can be prepared by reaction of the compound with an organic or inorganic acid, using standard methods detailed in the literature. Examples of useful salts include, but are not limited to, those prepared from the following acids: hydrochloric, hydrobromic, sulfuric, nitric, phosphoric, maleic, acetic, salicyclic, p-toluenesulfonic, tartaric, citric, methanesulfonic, formic, malonic, succinic, naphthalene-2-sulphonic and benzenesulphonic, and the like. Also, pharmaceutically acceptable salts can be prepared as alkaline metal or alkaline earth salts, such as sodium, potassium, or calcium salts of a carboxylic acid group.

Compositions of Conjugates of the Invention

In certain instances, due to incomplete conversions, less than 100% yields, and other unavoidable complications routinely encountered during chemical syntheses, exemplary compositions of four-arm conjugates are those wherein at least 80% of the four-arm conjugates in the composition have a structure encompassed by the formula,

wherein:

R is a residue of a polyol, polythiol or polyamine bearing at from 3 to about 50 hydroxyl, thiol or amino groups;

each R¹ is H or methyl;

each R² is H or methyl, with the provisio that R¹ and R² are not both H;

each Q is a linker (and, in one or more embodiments, a hydrolytically stable linker); each POLY¹ is a water-soluble, non-peptidic polymer;

each X is spacer moiety that optionally includes a releasable linkage (e.g., a hydrolyzable linkage, an enzymatically degradable linkage, and so forth); and

q is a positive integer from 3 to about 50 (e.g., 4),

and pharmaceutically acceptable salts and solvates thereof.

Pharmaceutical Compositions of Conjugates of the Invention

The invention provides pharmaceutical compositions, both for veterinary and for human medical use, which comprise one or more multi-armed polymer conjugates of the invention or a pharmaceutically acceptable salt thereof, with one or more pharmaceutically acceptable carriers, and optionally any other therapeutic ingredients, stabilizers, or the like. The carrier(s) must be pharmaceutically acceptable in the sense of being compatible with the other ingredients of the formulation and not unduly deleterious to the recipient thereof. The compositions of the invention may also include polymeric excipients/additives or carriers, e.g., polyvinylpyrrolidones, derivatized celluloses such as hydroxymethylcellulose, hydroxyethylcellulose, and hydroxypropylmethylcellulose, Ficolls (a polymeric sugar), hydroxyethylstarch (HES), dextrates (e.g., cyclodextrins, such as 2-hydroxypropyl-β-cyclodextrin and sulfobutylether-β-cyclodextrin), polyethylene glycols, and pectin. The compositions may further include diluents, buffers, binders, disintegrants, thickeners, lubricants, preservatives (including antioxidants), flavoring agents, taste-masking agents, inorganic salts (e.g., sodium chloride), antimicrobial agents (e.g., benzalkonium chloride), sweeteners, antistatic agents, surfactants (e.g., polysorbates such as “TWEEN 20” and “TWEEN 80,” and pluronics such as F68 and F88, available from BASF), sorbitan esters, lipids (e.g., phospholipids such as lecithin and other phosphatidylcholines, phosphatidylethanolamines, fatty acids and fatty esters, steroids (e.g., cholesterol)), and chelating agents (e.g., EDTA, zinc and other such suitable cations). Other pharmaceutical excipients and/or additives suitable for use in the compositions according to the invention are listed in “Remington: The Science & Practice of Pharmacy,” 19^(th) ed., Williams & Williams, (1995), and in the “Physician's Desk Reference,” 52^(nd) ed., Medical Economics, Montvale, N.J. (1998), and in “Handbook of Pharmaceutical Excipients,” Third Ed., Ed. A. H. Kibbe, Pharmaceutical Press, 2000.

The conjugates may be formulated in compositions including those suitable for oral, rectal, topical, nasal, ophthalmic, or parenteral (including intraperitoneal, intravenous, subcutaneous, or intramuscular injection) administration. The compositions may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. In general, the compositions are prepared by bringing the active compound into association with a liquid carrier to form a solution or a suspension, or alternatively, bringing the conjugate into association with formulation components suitable for forming a solid, optionally a particulate product, and then, if warranted, shaping the product into a desired delivery form. Solid formulations, when particulate, will typically comprise particles with sizes ranging from about 1 nanometer to about 500 microns. In general, for solid formulations intended for intravenous administration, particles will typically range from about 1 nm to about 10 microns in diameter. Particularly preferred are sterile, lyophilized compositions that are reconstituted in an aqueous vehicle prior to injection.

The amount of multi-armed polymer conjugate in the formulation will vary depending upon the specific active agent employed, its activity, the molecular weight of the conjugate, and other factors such as dosage form, target patient population, and other considerations, and will generally be readily determined by one skilled in the art. In practice, this will depending upon the particular conjugate, its activity, the severity of the condition to be treated, the patient, the stability of the formulation, and the like. Compositions will generally contain anywhere from about 1% by weight to about 99% by weight conjugate, typically from about 2% to about 95% by weight conjugate, and more typically from about 5% to 85% by weight conjugate, and will also depend upon the relative amounts of excipients/additives contained in the composition. More specifically, the composition will typically contain at least about one of the following percentages of conjugate: 2%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, or more by weight.

Compositions of the present invention suitable for oral administration may be provided as discrete units such as capsules, cachets, tablets, lozenges, and the like, each containing a predetermined amount of the conjugate as a powder or granules; or a suspension in an aqueous liquor or non-aqueous liquid such as a syrup, an elixir, an emulsion, a draught, and the like.

Formulations suitable for parenteral administration conveniently comprise a sterile aqueous preparation of the prodrug conjugate, which can be formulated to be isotonic with the blood of the recipient.

Nasal spray formulations comprise purified aqueous solutions of the multi-armed polymer conjugate with preservative agents and isotonic agents. Such formulations are preferably adjusted to a pH and isotonic state compatible with the nasal mucous membranes.

Formulations for rectal administration may be presented as a suppository with a suitable carrier such as cocoa butter, or hydrogenated fats or hydrogenated fatty carboxylic acids.

Ophthalmic formulations are prepared by a similar method to the nasal spray, except that the pH and isotonic factors are preferably adjusted to match that of the eye.

Topical formulations comprise the multi-armed polymer conjugate dissolved or suspended in one or more media such as mineral oil, petroleum, polyhydroxy alcohols or other bases used for topical formulations. The addition of other accessory ingredients as noted above may be desirable.

Pharmaceutical formulations are also provided which are suitable for administration as an aerosol, e.g., by inhalation. These formulations comprise a solution or suspension of the desired multi-armed polymer conjugate or a salt thereof. The desired formulation may be placed in a small chamber and nebulized. Nebulization may be accomplished by compressed air or by ultrasonic energy to form a plurality of liquid droplets or solid particles comprising the conjugates or salts thereof.

Methods of Use

The multi-armed polymer conjugates provided herein can be used to treat or prevent any condition (e.g., cancer) responsive to administration of the conjugate described herein.

The multi-arm polymer conjugates of the invention are particularly useful as anticancer agents, i.e., an agent that can reduce the growth of one or more tumors. Exemplary cancer types include, but are not limited to, breast cancer, ovarian cancer, colon cancer, colorectal cancer, prostate cancer, gastric cancer, malignant melanoma, small cell lung cancer, non-small cell lung cancer, thyroid cancers, kidney cancer, cancer of the bile duct, brain cancer, cancer of the head and neck, lymphomas, leukemias, rhabdomyosarcoma, and neuroblastoma.

Methods of administration comprise administering to a mammal in need thereof a therapeutically effective amount of a composition or formulation containing a multi-arm polymer conjugate as provided herein. A therapeutically effective dosage amount of any specific multi-arm polymer conjugate will vary from conjugate to conjugate, patient to patient, and will depend upon factors such as the condition of the patient, the activity of the particular active agent employed, the route of delivery, and condition being treated.

Methods of treatment also include administering a therapeutically effective amount of a composition or formulation comprising a multi-arm polymer conjugate as described herein with a second anticancer agent (such as, for example, 5-fluorouracil, leucovorin, avastin, cetuximab, panitumumab, xeloda, abraxane, cis-platin, carboplatin, gemeitabine, and alimpta.

The multi-aim polymer conjugate of the invention may be administered once or several times a day, preferably once a day or less. Illustrative dosing schedules include once per week, once every two weeks, or once every three weeks. In the instance of a maintenance dose, dosing may take place even less frequently than once every three weeks, such as once monthly. The duration of the treatment may be once per day for a period of from two to three weeks and may continue for a period of months or even years. The daily dose can be administered either by a single dose in the form of an individual dosage unit or several smaller dosage units or by multiple administration of subdivided dosages at certain intervals.

It is to be understood that while the invention has been described in conjunction with the preferred specific embodiments thereof, that the foregoing description as well as the examples that follow are intended to illustrate and not limit the scope of the invention. Other aspects, advantages and modifications within the scope of the invention will be apparent to those skilled in the art to which the invention pertains.

All articles, books, patents, patent publications and other publications referenced herein are incorporated by reference in their entireties. In the event of an inconsistency between the teachings of this specification and the art incorporated by reference, the meaning of the teachings in this specification shall prevail.

EXPERIMENTAL

The practice of the invention will employ, unless otherwise indicated, conventional techniques of organic synthesis and the like, which are within the skill of the art. Such techniques are fully described in the literature. Reagents and materials are commercially available unless specifically stated to the contrary. See, for example, M. B. Smith and J. March, March's Advanced Organic Chemistry: Reactions Mechanisms and Structure, 6th Ed. (New York: Wiley-Interscience, 2007), supra, and Comprehensive Organic Functional Group Transformations II, Volumes 1-7, Second Ed.: A Comprehensive Review of the Synthetic Literature 1995-2003 (Organic Chemistry Series), Eds. Katritsky, A. R., et al., Elsevier Science.

In the following examples, efforts have been made to ensure accuracy with respect to numbers used (e.g., amounts, temperatures, etc.) but some experimental error and deviation should be accounted for. Unless indicated otherwise, temperature is in degrees C. and pressure is at or near atmospheric pressure at sea level.

The following examples illustrate certain aspects and advantages of the present invention, however, the present invention is in no way considered to be limited to the particular embodiments described below.

Example 1 Synthesis of 4-ARM-PEG_(20K)-Butanoic Acid (“4-ARM-PEG_(20K)-BA”)

A solution of pentaerythritol-based 4-ARM-PEG_(20K)-OH (100.0 g, 0.020 equivalents) (NOF Corporation) in toluene (750 ml) was azeotropically dried by distilling off 150 ml of toluene. A 1.0M solution of potassium tert-butoxide in tert-butanol (60 ml, 0.060 moles) and 1-(3-bromopropyl)-4-methyl-2,6,7-trioxabicyclo[2,2,2]octane (12.6 g, 0.052 moles) was added and the mixture was stirred overnight at 70° C. under argon atmosphere. The solvent was distilled off under reduced pressure and the residue was dissolved in distilled water (1,000 ml). The pH of the solution was adjusted to 2 with 5% phosphoric acid and the solution was stirred for 15 minutes at room temperature. Next, the pH was readjusted to 12 by addition of 1M sodium hydroxide, and the solution was stirred for two hours keeping the pH at 12 by periodic addition of 1M sodium hydroxide. Thereafter, the pH was adjusted to 3 by addition of 5% phosphoric acid and the product was extracted with dichloromethane.

The extract was dried over anhydrous magnesium sulfate, filtered, and then added to isopropyl alcohol. The precipitated product was removed by filtration and dried under reduced pressure.

Yield 95.0 g. ¹H NMR (d₆-DMSO): δ1.72 ppm (q, CH₂ —CH₂—COO—), 2.24 ppm (t, —CH₂—COO—), 3.51 ppm (s, PEG backbone). Substitution=˜100% (meaning that, within the sensitivity of the method, the OH— groups located on the end of each arm of the 4-ARM-PEG starting material were converted to the corresponding butanoic acid).

Example 2 Synthesis of 4-ARM-PEG_(20K)-Butanoate-Linked Cabazitaxel Conjugate (“4-ARM-PEG_(20K)-BA-CAB”)

To a solution of 4-ARM-PEG_(20K)-butanoic acid (5.0 g, 0.0010 equivalents, Example 1), cabazitaxel (1.0 g, 0.0012 moles), and p-toluenosulfonic acid 4-dimethylaminopyridine salt (1.5 g, 0.0051 moles) in 60 ml of anhydrous dichloromethane was added N,N′-diisopropylcarbodiimide (0.63 g, 0.005 mole) and the mixture was stirred overnight at room temperature under an argon atmosphere. The solvent was distilled off under reduced pressure. The residue was dissolved in dichloromethane (7.5 ml) and added to a 1:1 mixture of isopropyl alcohol and diethyl ether (100 ml). The precipitated product was filtered off and dried under reduced pressure. The precipitation was repeated giving 3.8 g of white solid product.

NMR analysis of the product in CDCl₃ as a solvent showed that to each molecule of 4ARM-PEG_(20K)-butanoic acid (4-ARM-PEG_(20K)-BA) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.10 (d, 8H), 7.58 (m, 4H), 7.44 (m, 8H), 7.30 (m, 8H), 7.24 (m, 8H), 6.25 (t, 4H), 5.62 (d, 4H), 5.48 (m, 8H), 5.28 (s, 4H), 5.00 (d, 4H), 4.82 (s, 4H), 4.30 (d, 4H), 4.15 (d, 4H), 3.90 (m, 4H), 3.85 (d, 4H), 3.80-3.40 (m, 1891H), 3.41 (s, 3H), 3.30 (s, 3H), 2.70 (m, 4H), 2.52 (m, 4H), 2.43 (m, 14H), 2.30 (m, 4H), 2.15 (m, 4H), 2.00 (s, 12H), 1.85 (m, 8H), 1.80 (m, 41-1), 1.70 (s, 12H), 1.35 (s, 36H), 1.19 (m, 24H).

Example 3 Synthesis of 4-ARM-PEG_(20K)-Acetic Acid“4-ARM-PEG_(20K)-CM”)

A solution of pentaerythritol-based 4-ARM-PEG_(20K)-OH (100.0 g, 0.020 equivalents) (NOF Corporation) in toluene (750 ml) was azeotropically dried by distilling off 150 ml of toluene. 1.0M solution of potassium tert-butoxide in tert-butanol (60 ml, 0.060 moles) and tert-butyl bromoacetate (12.9 g, 0.066 moles) were added and the mixture was stirred overnight at 45° C. under argon atmosphere. The solvent was distilled off under reduced pressure and the residue was dissolved in distilled water (1,000 ml). The pH of the solution was adjusted to 12 by addition of 1M sodium hydroxide and the solution was stirred overnight keeping the pH at 12.0 by periodic addition of 1M sodium hydroxide. The pH was adjusted to 2 by addition of 1M phosphoric acid. The resulting product, 4-ARM-PEG_(20K)-acetic acid (also referred to as “4-ARM-PEG_(20K)-CM”) was extracted with dichloromethane. The extract was dried over anhydrous sodium sulfate, filtered, and concentrated. The concentrated extract was then added to ethyl ether.

The precipitated product was collected by filtration and dried under reduced pressure. Yield 95.5 g. ¹H NMR (d₆-DMSO): δ 3.51 ppm (s, PEG backbone), 4.01 ppm (s, —CH₂—COO—). Substitution ˜100% (meaning that, within the sensitivity of the method, the OH— groups located on the ends of each arm of the 4-ARM-PEG starting material was converted to the corresponding acetic acid).

Example 4 Synthesis of 4-ARM-PEG₂₀-Acetic Acid, N-Hydroxysuccinimide ester (“4-ARM-PEG_(20K)-SCM”)

4-ARM-PEG_(20K)-acetic acid (90.0 g, 0.018 equivalents) was dissolved in dichloromethane (270 ml) and N-hydroxysuccinimide (2.20 g, 0.019 mol) was added. Next, dicyclohexylcarbodiimide (4.13 g, 0.020 moles) was added, and the solution was stirred at room temperature overnight. The reaction mixture was filtered, concentrated, and precipitated by addition to isopropyl alcohol.

Yield of final product: 82 g. ¹H NMR (d₆-DMSO): δ 2.81 ppm (s, succinimide), 3.51 ppm (s, PEG backbone), 4.60 ppm (s, —CH₂—COO—). Substitution ˜100%.

Example 5 Synthesis of 4-ARM-PEG_(20K)-Acetate-Linked Compound B Conjugate (“4-ARM-PEG_(20K)-CM-10-MDOC”)

To a solution of 4-ARM-PEG_(20K)-acetic acid (5.0 g, 0.0010 equivalents, Example 3), Compound B (1.0 g, 0.0012 moles), and p-toluenosulfonic acid 4-dimethylaminopyridine salt (1.5 g, 0.0051 moles) in 60 ml of anhydrous dichloromethane, was added N,N′-diisopropylcarbodiimide (0.63 g, 0.005 mole) and the mixture was stirred overnight at room temperature under an argon atmosphere. The solvent was distilled off under reduced pressure. The residue was dissolved in dichloromethane (7.5 ml) and added to a 1:1 mixture of isopropyl alcohol and diethyl ether (100 ml). The precipitated product was filtered off and dried under reduced pressure. The precipitation was repeated giving 3.7 g of white solid product.

NMR analysis of the product in CDCl₃ as a solvent showed that to each molecule of 4ARM-PEG_(20K)-acetic acid (4-ARM-PEG_(20K)-CM) was connected ˜4 molecules of Compound B.

Example 6 Synthesis of Cabazitaxel-2′-O-Alaninate

N-CBZ-(D,L)-Alanine (0.52 g, 0.00233 mol) was dissolved in 200 mL of anhydrous dichloromethane at room temperature. To this solution, N,N′-diisopropylcarbodiimide (DIPC; 0.75 ml 0.00469 mol), 4-dimethylaminopyridine (DMAP; 0.38 g, 0.00313 mol), and cabazitaxel (1.31 g, 0.00157 mol) were added at 0° C. Then, the reaction mixture was stirred overnight at room temperature under a nitrogen atmosphere. The solution was washed with 0.1N HCl, dried and concentrated to dryness under reduced pressure. The crude product (1.72 g) was purified by silica gel chromatography to give 1.4 g of Cabazitaxel-2′-ester of N-CBZ-(D,L)-Alanine.

¹H NMR (CDCl₃): δ 8.13 (d, 2H), 7.63 (m, 1H), 7.51 (m, 2H), 7.35 (m, 10H), 6.27 (t, 1H), 5.65 (d, 1H), 5.52 (br., 1H), 5.42 (m, 1H), 5.35 (s, 1H), 5.25 (d, 1H), 5.10 (m, 2H), 5.00 (d, 1H), 4.82 (s, 1H), 4.50 (m, 1H), 4.32 (d, 1H), 4.20 (d, 1H), 3.90 (m, 2H), 3.45 (s, 3H), 3.30 (s, 3H), 2.72 (m, 1H), 2.46 (s, 3H), 2.32 (m, 1H), 2.22 (m, 1H), 2.00 (s, 3H), 1.80 (t, 1H), 1.72 (s, 3H), 1.36 (s, 9H), 1.24 (m, 9H). LC-MS (m/z): Calculated 1040.5; found 1041.5 [M+H]

Removal of CBZ protective group was achieved by hydrogenation (hydrogen pressure 40 psi, five hours) performed in tetrahydrofuran (THF; 20 ml), in the presence of hydrogenation catalyst (10% of palladium hydroxide on the active carbon; 0.14 g). The crude product was purified by silica gel chromatography giving the desired cabazitaxel-2′-O-alaninate (0.95 g). ¹H NMR (CDCl₃): δ 8.13 (d, 2H), 7.63 (m, 1H), 7.51 (m, 2H), 7.40 (m, 2H), 7.30 (m, 3H), 6.27 (t, 1H), 5.52 (br., 1H), 5.45 (m, 2H), 5.00 (d, 1H), 4.85 (s, 1H), 4.32 (d, 1H), 4.20 (d, 1H), 3.90 (m, 1H), 3.85 (m, 1H), 3.65 (m, 1H), 3.45 (s, 3H), 3.30 (s, 3H), 2.72 (m, 1H), 2.46 (s, 3H), 2.32 (m, 1H), 2.22 (m, 1H), 2.00 (s, 3H), 1.80 (t, 1H), 1.72 (s, 3H), 1.36 (s, 9H), 1.22 (d, 6H), 1.15 (d, 3H). LC-MS (m/z): Calculated. 906.4; found 907.5 [M+H]⁺.

Example 7 Synthesis of Cabazitaxel-2′-O-Glycinate

The procedure of Example 6 was repeated using the same molar amount of N-CBZ-(D,L)-Glycine instead of N-CBZ-(D,L)-Alanine. The cabazitaxel-2′-O-glycinate (0.85 g) obtained was analyzed by NMR and LC-MS: ¹H NMR (CDCl₃): δ 8.13 (d, 2H), 7.62 (m, 1H), 7.51 (m, 2H), 7.42 (m, 2H), 7.35 (m, 1H), 7.30 (m, 2H), 6.25 (t, 1H), 5.50 (br., 1H), 5.40 (s, 1H), 5.35 (m, 1H), 5.00 (d, 1H), 4.85 (s, 1H), 4.34 (d, 1H), 4.20 (d, 1H), 3.90 (m, 1H), 3.85 (m, 1H), 3.60 (d, 1H), 3.45 (s, 3H), 3.30 (s, 3H), 2.72 (m, 1H), 2.45 (s, 3H), 2.32 (m, 1H), 2.22 (m, 1H), 2.00 (s, 3H), 1.80 (t, 1H), 1.70 (s, 3H), 1.36 (s, 9H), 1.22 (d, 6H). LC-MS (m/z): Calculated. 892.4; found 893.3 [M+H]⁺.

Example 8 Synthesis of 4-ARM-PEG_(20K)-Alaninate-Linked Cabazitaxel Conjugate (“4-ARM-PEG_(20K)-CM-ALA-CAB”)

4-ARM-PEG_(20K)-acetic acid, N-hydroxysuccinimide ester (3.70 g, 0.000740 equivalents; Example 4) was added slowly to the solution of cabazitaxel-2′-O-alaninate (0.80 g, 0.000882 moles) in dichloromethane (40 ml) containing triethylamine (0.35 g). The solution was stirred at room temperature overnight. The reaction mixture was concentrated to dryness by distilling off dichloromethane under reduced pressure. The crude product was dissolved in 120 ml dichloromethane and precipitated by addition of isopropyl alcohol.

The precipitate, (4-ARM-PEG_(20K)-CM-ALA-CAB) was collected and dried to provide 3.4 g of a white solid product. NMR analysis of the product in CDCl₃ as a solvent showed that to each molecule of 4ARM-PEG_(20K)-alaninate (4-ARM-PEG_(20K)-CM-ALA) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.12 (d, 8H), 7.60 (m, 4H), 7.50 (m, 8H), 7.40 (m, 12H), 7.30 (m, 8H), 6.25 (t, 4H), 5.65 (m, 8H), 5.50 (br., 4H), 5.38 (s, 4H), 5.00 (d, 4H), 4.82 (s, 4H), 4.70 (m, 4H), 4.30 (d, 4H), 4.15 (d, 4H), 4.00 (d, 4H), 3.90 (m, 4H), 3.85 (m, 8H), 3.80 (m, 8H), 3.76-3.46 (m, 1926H), 3.40 (s, 12H), 3.30 (s, 12H), 2.72 (m, 4H), 2.42 (s, 12H), 2.32 (m, 4H), 2.22 (m, 4H), 2.00 (s, 12H), 1.80 (t, 4H), 1.70 (s, 12H), 1.35 (m, 48H), 1.20 (d, 24H).

Example 9 Synthesis of 4-ARM-PEG_(20K)-Glycinate-Linked Cabazitaxel Conjugate (“4-ARM-PEG_(20K)-CM-GLY-CAB”)

The procedure of Example 8 was repeated using, instead of cabazitaxel-2′-β-alaninate and triethylamine, the same molar amounts of cabazitaxel-2′-O-glycinate and diisopropylethylamine. NMR analysis of the obtained product showed that to each molecule of 4ARM-PEG_(20K)-glycinate (4-ARM-PEG_(20K)-CM-GLY) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.12 (d, 8H), 7.70 (br., 4H), 7.60 (m, 4H), 7.50 (m, 8H), 7.40 (m, 8H), 7.30 (m, 8H), 6.25 (t, 4H), 5.80 (br., 4H), 5.65 (d, 4H), 5.50 (br., 4H), 5.38 (s, 4H), 5.00 (d, 4H), 4.85 (s, 4H), 4.30 (d, 4H), 4.22 (d, 4H), 4.15 (m, 8H), 4.00 (d, 4H), 3.90 (m, 4H), 3.85 (m, 8H), 3.80 (m, 8H), 3.70-3.50 (m, 1984H), 3.45 (s, 12H), 3.30 (s, 12H), 2.72 (m, 4H), 2.45 (s, 12H), 2.32 (m, 4H), 2.22 (m, 4H), 2.00 (s, 12H), 1.80 (t, 4H), 1.70 (s, 12H), 1.35 (s, 36H), 1.20 (d, 24H)

Example 10 Synthesis of 4-ARM-PEG_(30K)-Glycinate-Linked Cabazitaxel Conjugate (“4-ARM-PEG_(30K)-CM-GLY-CAB”)

The procedure of Example 9 was repeated using, instead of 4-ARM-PEG_(20K)-acetic acid, N-hydroxysuccinimide ester, the same molar amount of 4-ARM-PEG_(30K)-acetic acid, N-hydroxysuccinimide ester. NMR analysis of the product showed that to each molecule of 4ARM-PEG_(30K)-glycinate (4-ARM-PEG_(30K)-CM-GLY) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.11 (d, 8H), 7.70 (br., 4H), 7.60 (m, 4H), 7.50 (m, 8H), 7.40 (m, 8H), 7.30 (m, 8H), 6.25 (t, 4H), 5.80 (br., 4H), 5.65 (d, 4H), 5.50 (br., 4H), 5.38 (s, 4H), 5.00 (d, 4H), 4.85 (s, 4H), 4.30 (d, 4H), 4.22 (d, 4H), 4.15 (m, 8H), 4.00 (d, 4H), 3.90-3.46 (m, 3177H), 3.45 (s, 12H), 3.30 (s, 12H), 2.72 (m, 4H), 2.45 (s, 12H), 2.32 (m, 4H), 2.22 (m, 4H), 2.00 (s, 12H), 1.80 (t, 4H), 1.70 (s, 12H), 1.35 (s, 36H), 1.20 (d, 24H).

Example 11 Synthesis of 4-ARM-PEG_(40K)-Glycinate-Linked Cabazitaxel Conjugate (“4-ARM-PEG_(40K)-CM-GLY-CAB”)

The procedure of Example 10 was repeated using, instead of 4-ARM-PEG_(30K)-acetic acid, N-hydroxysuccinimide ester, the same molar amount of 4-ARM-PEG_(40K)-acetic acid, N-hydroxysuccinimide ester. NMR analysis of the product showed that to each molecule of 4ARM-PEG_(40K)-glycinate (4-ARM-PEG_(40K)-CM-GLY) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.11 (d, 8H), 7.70 (br., 4H), 7.60 (m, 4H), 7.50 (m, 8H), 7.40 (m, 8H), 7.30 (m, 8H), 6.25 (t, 4H), 5.80 (br., 4H), 5.65 (d, 4H), 5.50 (br., 4H), 5.38 (s, 4H), 5.00 (d, 4H), 4.85 (s, 4H), 4.30 (d, 4H), 4.22 (d, 4H), 4.15 (m, 8H), 4.00 (d, 4H), 3.90 (m, 4H), 3.85 (m, 8H), 3.70-3.50 (m, 4179H), 3.45 (s, 12H), 3.30 (s, 12H), 2.72 (m, 4H), 2.45 (s, 12H), 2.32 (m, 4H), 2.22 (m, 4H), 2.00 (s, 12H), 1.80 (t, 4H), 1.70 (s, 12H), 1.35 (s, 36H), 1.20 (d, 24H).

Example 12 Synthesis of 4-ARM-PEG_(30K)-Alaninate-Linked Cabazitaxel Conjugate (“4-ARM-PEG_(30K)-CM-ALA-CAB”)

The procedure of Example 8 was repeated using, instead of 4-ARM-PEG_(20K)-acetic acid, N-hydroxysuccinimide ester, the same molar amount of 4-ARM-PEG_(30K)-acetic acid, N-hydroxysuccinimide ester. NMR analysis of the obtained product showed that to each molecule of 4ARM-PEG_(30K)-alaninate (4-ARM-PEG_(30K)-CM-ALA) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.10 (d, 8H), 7.60 (m, 4H), 7.50 (m, 8H), 7.40 (m, 12H), 7.30 (m, 8H), 6.25 (t, 4H), 5.65 (m, 8H), 5.50 (br., 4H), 5.38 (s, 4H), 5.00 (d, 4H), 4.82 (s, 4H), 4.70 (m, 4H), 4.30 (d, 4H), 4.15 (d, 4H), 4.00 (d, 4H), 3.90 (m, 4H), 3.85 (m, 8H), 3.80 (m, 8H), 3.76-3.46 (m, 3108H), 3.40 (s, 12H), 3.30 (s, 12H), 2.72 (m, 4H), 2.42 (s, 12H), 2.32 (m, 4H), 2.22 (m, 4H), 2.00 (s, 12H), 1.80 (t, 4H), 1.70 (s, 12H), 1.35 (m, 48H), 1.20 (d, 24H).

Example 13 Synthesis of 4-ARM-PEG_(40K)-Alaninate-Linked Cabazitaxel Conjugate (“4-ARM-PEG_(40K)-CM-ALA-CAB”)

The procedure of Example 12 was repeated using, instead of 4-ARM-PEG_(30K)-acetic acid, N-hydroxysuccinimide ester, the same molar amount of 4-ARM-PEG_(40K)-acetic acid, N-hydroxysuccinimide ester. NMR analysis of the product obtained showed that to each molecule of 4ARM-PEG_(30K)-glycinate (4-ARM-PEG_(30K)-CM-GLY) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.11 (d, 8H), 7.60 (m, 4H), 7.50 (m, 8H), 7.40 (m, 12H), 7.30 (m, 8H), 6.25 (t, 4H), 5.65 (m, 8H), 5.50 (br., 4H), 5.38 (s, 4H), 5.00 (d, 4H), 4.82 (s, 4H), 4.70 (m, 4H), 4.30 (d, 4H), 4.15 (d, 4H), 4.00 (d, 4H), 3.90 (m, 4H), 3.85 (m, 8H), 3.80-3.46 (m, 4183H), 3.40 (s, 12H), 3.30 (s, 12H), 2.72 (m, 4H), 2.42 (s, 12H), 2.32 (m, 4H), 2.22 (m, 4H), 2.00 (s, 12H), 1.80 (t, 4H), 1.70 (s, 12H), 1.35 (m, 48H), 1.20 (d, 24H).

Example 14 Synthesis of 4-ARM-PEG_(20K)-Acetate-Linked Cabazitaxel (“4-ARM-PEG_(20K)-CM-CAB”)

The procedure of Example 5 was repeated using, instead of Compound B. the same molar amount of cabazitaxel. NMR analysis of the obtained product showed that to each molecule of 4ARM-PEG_(20K)-acetic acid (4-ARM-PEG_(20K)-CM) was connected ˜4 molecules of cabazitaxel. ¹H NMR (CDCl₃): δ 8.12 (d, 8H), 7.60 (m, 4H), 7.50 (m, 8H), 7.40 (m, 8H), 7.26 (m, 8H), 6.25 (t, 4H), 5.70 (br., 4H), 5.62 (d, 4H), 5.50 (br., 4H), 5.35 (s, 4H), 5.00 (d, 4H), 4.80 (s, 4H), 4.30 (m, 8H), 4.15 (m, 4H), 3.85 (m, 8H), 3.80-3.48 (m, 1998H), 3.40 (s, 3H), 3.30 (s, 3H), 2.70 (m, 4H), 2.45 (s, 12H), 2.35 (m, 4H), 2.25 (m, 4H), 2.00 (s, 12H), 1.80 (m, 4H), 1.70 (s, 12H), 1.32 (s, 36H), 1.21 (s, 24H).

Example 15 Maximum Tolerated Doses (MTD) of PEGylated-Conjugates of Cabazitaxel in Athymic Mice

Purpose. This study was performed to determine appropriate doses of each compound for tumor xenograft studies. In those studies, it is desirable to administer the maximum dose of test compound that will not cause excessive loss of body weight. In general, loss of ˜15% of the initial body weight of the animal is typically the maximum desired.

Experimental procedure. MTD for 4-arm-conjugated to cabazitaxel were determined in female athymic nu/nu mice using standard methods. Briefly, 8-12 week old mice were administered test compounds or cabazitaxel intravenously at one week intervals for a total of three doses per mouse (qw×3, iv) as shown in Tables 1 and 2. For all PEG-conjugated test compounds, the doses of both the conjugate and released cabazitaxel, calculated from the cabazitaxel content of the respective conjugate, are shown. Cabazitaxel solutions were prepared in D5W containing 5% Tween 80:10% ethanol. PEG-cabazitaxel conjugates were prepared in D5W. The administration volume for all test articles was 10 mL/kg. Mice were observed daily for clinical signs and body weights were recorded on days 1-5 and then biweekly until the end of study. All procedures were conducted in compliance with IACUC requirements. Any individual animal with a single observation of greater than 30% body weight loss or three consecutive measurements of greater than 25% body weight loss was euthanized. For any group with two measurements of mean body weight loss of greater than 20%, dosing was stopped and recovery was allowed. Within a group with greater than 20% mean weight loss, individual animals greater than 30% body weight loss were euthanized. Compound dosing was terminated for any group in which mean weight loss exceeded 20% or >10% of animals died. Moribund animals were euthanized and all animals were euthanized at end of study (day 30).

TABLE 1 MTS Study Treatment Plan (“20K Conjugates”) Cabazitaxel Schedule, Group N Agent mg/kg equivalent mg/kg route 1 5 Cabazitaxel 30 30 Qw x 3, iv 2 5 4-arm-PEG_(20K)-CM-Cabazitaxel 57.16 7.5 Qw x 3, iv 3 5 4-arm-PEG_(20K)-CM-Cabazitaxel 114.33 15 Qw x 3, iv 4 5 4-arm-PEG_(20K)-CM-Cabazitaxel 228.66 30 Qw x 3, iv 5 5 4-arm-PEG_(20K)-BA-Cabazitaxel 66.25 7.5 Qw x 3, iv 6 5 4-arm-PEG_(20K)-BA-Cabazitaxel 132.51 15 Qw x 3, iv 7 5 4-arm-PEG_(20K)-BA-Cabazitaxel 265.02 30 Qw x 3, iv 8 5 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 57.34 7.5 Qw x 3, iv 9 5 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 114.68 15 Qw x 3, iv 10 5 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 229.36 30 Qw x 3, iv 11 5 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 56.01 7.5 Qw x 3, iv 12 5 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 112.02 15 Qw x 3, iv 13 5 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 224.05 30 Qw x 3, iv

TABLE 2 MTS Study Treatment Plan (“30K and 40K Conjugates”) Cabazitaxel Schedule, Group N Agent mg/kg equivalent mg/kg route 1 5 Cabazitaxel 30 30 Qw x 3, iv 2 5 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 85.91 7.5 Qw x 3, iv 3 5 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 171.82 15 Qw x 3, iv 4 5 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 343.64 30 Qw x 3, iv 5 5 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 84.18 7.5 Qw x 3, iv 6 5 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 168.35 15 Qw x 3, iv 7 5 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 336.7 30 Qw x 3, iv 8 5 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 110.62 7.5 Qw x 3, iv 9 5 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 221.24 15 Qw x 3, iv 10 5 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 442.48 30 Qw x 3, iv 11 5 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 109.81 7.5 Qw x 3, iv 12 5 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 219.62 15 Qw x 3, iv 13 5 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 439.24 30 Qw x 3, iv

Results. The results for the study MTD “20K Conjugates” are summarized in Table 3 and FIG. 1 while the results for the MTD “30K and 40K Conjugates” study are summarized Table 4 and FIG. 2. Based on these data and the data for individual animals within the test groups, the estimated MTD of each compound is provided in Table 5. It should be noted that this is not meant to be a definitive MTD, but is simply a guide to select an appropriate dose for conducting tumor xenograft studies using this administration route and schedule. Note too, that these MTD are given as cabazitaxel equivalents. The actual dose of each conjugate must be corrected for the wt % of cabazitaxel in that specific conjugate.

TABLE 3 MTD Results for “20K Conjugates” Treatment - Mean Cabazitaxel BW Nadir Related Day of Group N Agent eq. mg/kg (day) Deaths Death 1 5 Cabazitaxel 30 −15.4% (20) 0 — 2 5 4-arm-PEG_(20K)-CM-Cabazitaxel 7.5 −3.4% (3) 0 — 3 5 4-arm-PEG_(20K)-CM-Cabazitaxel 15 −2.0% (3) 0 — 4 5 4-arm-PEG_(20K)-CM-Cabazitaxel 30 −10.2% (23) 0 — 5 5 4-arm-PEG_(20K)-BA-Cabazitaxel 7.5 — 0 — 6 5 4-arm-PEG_(20K)-BA-Cabazitaxel 15 −1.3% (2) 0 — 7 5 4-arm-PEG_(20K)-BA-Cabazitaxel 30 −16.6% (20) 0 — 8 5 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 7.5 −0.7% (3) 0 — 9 5 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 15 — 0 — 10 5 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 30 −15.6% (23) 0 — 11 5 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 7.5  −4.6% (13) 1 15 12 5 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 15 −4.4% (3) 0 — 13 5 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 30 −22.1% (23) 0 —

TABLE 4 MTD Results for 30K and 40K Conjugates Cabazitaxel Treatment- Mean eq. BW Nadir Related Day of Group N Agent mg/kg (day) Deaths Death 1 5 Cabazitaxel 30  −6.8% (21) 0 — 2 5 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 7.5 −0.2% (2) 0 — 3 5 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 15  −3.7% (11) 0 — 4 5 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 30 −23.5% (24) 0 — 5 5 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 7.5 −2.7% (2) 0 — 6 5 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 15 −15.2% (21) 0 — 7 5 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 30 −30.9% (21) 0 — 8 5 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 7.5 −3.8% (2) 0 — 9 5 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 15 −6.7% (5) 0 — 10 5 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 30  −6.2% (21) 0 — 11 5 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 7.5 — 0 — 12 5 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 15  −4.4% (21) 0 — 13 5 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 30 −25.9% (21) 1 23

TABLE 5 Estimated MTD for Exemplary PEG-Cabazitaxel Conjugates MTD, mg/kg, Agent cabazitaxel equivalents 4-arm-PEG_(20K)-CM-Cabazitaxel 25 4-arm-PEG_(20K)-BA-Cabazitaxel 25 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 20 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 20 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 20 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 12 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 30 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 20

Example 16 Inhibition of Tumor Xenograft Growth by PEGylated-Conjugates of Cabazitaxel in Athymic Mice

Purpose. Studies were conducted to assess the growth inhibitory effects of PEGylated-conjugates of cabazitaxel on H460 non-small cell lung cancer (NSCLC) implants in athymic nu/nu mice.

Experimental plan. These studies were conducted using routine methods and materials that are familiar to those skilled in the art. Briefly, 8 to 12 week old female nu/nu mice were injected subcutaneously in the flank with 1×10⁷ H460 tumor cells in 0.2 mL sterile buffer. When tumors reached an average size of 80-120 mm³, animals were assigned to treatment groups to provide similar tumor size distributions within each group and treatment was begun. Animals received three intravenous doses of test article at one week intervals beginning on day 1. Cabazitaxel solutions were prepared in D5W containing 5% Tween 80:10% ethanol. PEG-cabazitaxel conjugates were prepared in D5W. The administration volume for all test articles was 10 mL/kg. The test agents and concentrations are shown in Tables 6 and 7. The test doses were selected to represent the MTD and ½ the MTD for each test compound. The tables list the actual mg/kg for each test article and the corresponding cabazitaxel equivalent calculated based on the weight-percent cabazitaxel content for each PEGylated conjugate.

Animals were weighed on days 1-5, 7, 9, 11, 13 then biweekly until the end of the study. Tumor sizes were measured using calipers biweekly throughout the study and tumor volumes were calculated using the formula for an ellipsoid sphere: L×W²/2=mm³, where L and W are the major axis dimensions of the tumor in mm. Additional parameters that were monitored were the number of non-specific deaths, number of partial and complete tumor regressions, number of tumor-free survivors, and the time required for individual animals' tumors to reach 2000 mm³. The median time for a tumor to reach 2000 mm³ in the treatment groups (T) and control group (C) was used to calculate of the overall delay in the growth of the median tumor (TGD). Animals were monitored individually until reaching the experimental endpoint: tumor size of 2000 mm³ or 45 days, whichever came first. Animals in which tumors do not reach 2000 mm³ within 45 days will be followed longer in order to determine tumor growth delay. When the study endpoint was reached, the animals were euthanized.

TABLE 6 Test Agents and Concentrations for 20K Conjugate Xenograft Study Cabazitaxel Group N Test compound mg/kg eq. mg/kg Route Schedule 1 10 Vehicle — — iv qwk x 3 2 10 Cabazitaxel 15 15 iv qwk x 3 3 10 Cabazitaxel 30 30 iv qwk x 3 4 10 4-arm-PEG_(20K)-CM-Cabazitaxel 95.3 12.5 iv qwk x 3 5 10 4-arm-PEG_(20K)-CM-Cabazitaxel 190.6 25 iv qwk x 3 6 10 4-arm-PEG_(20K)-BA-Cabazitaxel 91.3 12.5 iv qwk x 3 7 10 4-arm-PEG_(20K)-BA-Cabazitaxel 182.6 25 iv qwk x 3 8 10 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 76.5 10 iv qwk x 3 9 10 4-arm-PEG_(20K)-CM-glycine-Cabazitaxel 152.9 20 iv qwk x 3 10 10 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 74.7 10 iv qwk x 3 11 10 4-arm-PEG_(20K)-CM-alanine-Cabazitaxel 149.4 20 iv qwk x 3

TABLE 7 Test Agents and Concenrations for the 30K and 40K Conjugate Xenograft Study Cabazitaxel Group N Test compound mg/kg eq. mg/kg Route Schedule 1 10 Vehicle — — iv qwk x 3 2 10 Cabazitaxel 15 15 iv qwk x 3 3 10 Cabazitaxel 30 30 iv qwk x 3 4 10 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 114.6 10 iv qwk x 3 5 10 4-arm-PEG_(30K)-CM-glycine-Cabazitaxel 229.1 20 iv qwk x 3 6 10 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 67.3 6 iv qwk x 3 7 10 4-arm-PEG_(30K)-CM-alanine-Cabazitaxel 134.7 12 iv qwk x 3 8 10 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 221.2 15 iv qwk x 3 9 10 4-arm-PEG_(40K)-CM-glycine-Cabazitaxel 442.5 30 iv qwk x 3 10 10 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 146.4 10 iv qwk x 3 11 10 4-arm-PEG_(40K)-CM-alanine-Cabazitaxel 292.8 20 iv qwk x 3

Results. The results for the “20K conjugate” xenograft study through study day 47 are shown in Table 8 and FIG. 3. In FIG. 3, arrows indicate treatment administrations. Data is not shown for treatment groups after >20% of test animals exceed the maximum allowable tumor volume. No animal deaths were reported during this study. Weight loss was within acceptable ranges, although animals treated with 4-arm-PEG_(20K)-BA-cabazitaxel at 25 mg/kg exhibited slightly higher than expected weight loss. Animals reached their maximum weight loss 3-8 days following the third dose of the test articles. Partial responses were recorded among animals treated with Cabazitaxel (3 animals, 30 mg/kg), 4-arm-PEG_(20K)-CM-cabazitaxel (10 animals, 25 mg/kg), and 4-arm-PEG_(20K)-CM-glycine-cabazitaxel (4 animals, 20 mg/kg). Despite relatively greater toxicity, as suggested by greater weight loss, 4-arm-PEG_(20K)-BA-Cabazitaxel did not provide greater tumor growth inhibition than either cabazitaxel or the other PEG-cabazitaxel conjugates tested in this study.

TABLE 8 Summary Results for 20K Conjugate Xenograft Study (Through Day 46) MTV BW Cabazitaxel Median TGD, % mm³, Nadir Test Article eq. mg/kg TTE, d d TGD (n) PR CR TFS (d) Deaths Vehicle — 18.5 — — — 0 0 0 — 0 Cabazitaxel 15 28.1 9.6 52 1764 0 0 0 −15.0% 0 (1) (22) Cabazitaxel 30 NR 28.5 154 918 3 0 0 −6.4% 0 (8) (22) 4-arm-PEG_(20K)- 12.5 36.8 18.3 99 — 0 0 0 −15.9% 0 CM-Cabazitaxel (22) 4-arm-PEG_(20K)- 25 NR 28.5 154 358 10 0 0 −10.1% 0 CM-Cabazitaxel (10) (22) 4-arm-PEG_(20K)-BA- 12.5 25.3 6.7 36 — 0 0 0 −4.3% 0 Cabazitaxel (22) 4-arm-PEG_(20K)-BA- 25 39.3 20.8 112 — 0 0 0 −19.6% 0 Cabazitaxel (22) 4-arm-PEG_(20K)- 10 30.1 11.6 63 1200 0 0 0 −9.2% 0 CM-glycine- (2) (22) Cabazitaxel 4-arm-PEG_(20K)- 20 NR 28.5 154 1183 4 0 0 −9.5% 0 CM-glycine- (9) (22) Cabazitaxel 4-arm-PEG_(20K)- 10 35.6 17.1 93 908 0 0 0 −6.6% 0 CM-alanine- (1) (22) Cabazitaxel 4-arm-PEG_(20K)- 20 NR 28.5 154 1470 0 0 0 −13.0% 0 CM-alanine- (7) (17) Cabazitaxel Median TTE: Median Time to Endpoint; NR = not reached; TGD: Mean tumor growth delay compared to vehicle; MTV (n): Median Tumor Volume (number of animals used for calculation); PR: Number of Partial Regressions; CR: Number of Complete Regressions; TFS: Number of Tumor Free Survivors; BW Nadir: Lowest Mean Body Weight for Group (based on at least half of the remaining animals, expressed as % of initial mean BW).

The results for the “30K and 40K conjugate” xenograft study through study day 45 are shown in Table 9 and FIG. 4. In FIG. 4, arrows indicate treatment administrations. Data is not shown for treatment groups after >20% of test animals exceed the maximum allowable tumor volume. Weight loss was within acceptable ranges and no animal deaths were reported during this study. Animals reached their maximum weight loss 4-11 days following the third dose of the test articles. Partial responses were recorded among animals treated with cabazitaxel (5 animals, 30 mg/kg), 4-arm-PEG_(30K)-CM-glycine-cabazitaxel (1 animal, 10 mg/kg; 6 animals, 20 mg/kg), 4-arm-PEG_(40K)-CM-glycine-cabazitaxel (10 animals, 30 mg/kg) and 4-arm-PEG_(40K)-CM-alanine-cabazitaxel (4 animals, 20 mg/kg). In addition, complete regression, i.e., no detectable tumor through day 45 of the study, was observed for one animal in each group treated with 4-arm-PEG_(30K)-CM-glycine-cabazitaxel, 4-arm-PEG_(40K)-CM-glycine-cabazitaxel, and 4-arm-PEG_(40K)-CM-alanine-cabazitaxel.

TABLE 9 Summary Results for 30K and 40K Conjugate Xenograft Study (Through Day 46) MTV BW Cabazitaxel Median TGD, % mm³, Nadir Test Article eq. mg/kg TTE, d d TGD (n) PR CR TFS (d) Deaths Vehicle — 14.9 — — — 0 0 0 — 0 Cabazitaxel 15 27.9 13 87 — 0 0 0 −11.3% 0 (22) Cabazitaxel 30 NR 31.1 208 994 5 0 0 −11.4% 0 (8) (22) 4-arm-PEG_(30K)-CM- 10 32.7 17.8 119 726 1 0 0 −5.9% 0 glycine-Cabazitaxel (1) (22) 4-arm-PEG_(30K)-CM- 20 NR 31.1 208 478 6 1 1 −9.8% 0 glycine-Cabazitaxel (10) (22) 4-arm-PEG_(30K)-CM- 6 20.8 5.9 39 — 0 0 0 −3.3% 0 alanine-Cabazitaxel (18) 4-arm-PEG_(30K)-CM- 12 38.6 23.6 158 600 0 0 0 −8.5% 0 alanine-Cabazitaxel (1) (22) 4-arm-PEG_(40K)-CM- 15 36.8 21.9 146 1632 0 0 0 −6.4% 0 glycine-Cabazitaxel (2) (22) 4-arm-PEG_(40K)-CM- 30 NR 31.1 208 32 9 1 1 −15.3% 0 glycine-Cabazitaxel (10) (22) 4-arm-PEG_(40K)-CM- 10 30.1 15.1 101 — 0 0 0 −9.5% 0 alanine-Cabazitaxel (25) 4-arm-PEG_(40K)-CM- 20 NR 31.1 208 184 4 1 1 −17.2% 0 alanine-Cabazitaxel (10) (18) Median TTE: Median Time to Endpoint NR = not reached; TGD: Mean tumor growth delay compared to vehicle; MTV (n): Median Tumor Volume (number of animals used for calculation); PR: Number of Partial Regressions; CR: Number of Complete Regressions; TFS: Number of Tumor Free Survivors; BW Nadir: Lowest Mean Body Weight for Group (based on at least half of the remaining animals, expressed as % of initial mean BW)

The invention(s) set forth herein has been described with respect to particular exemplified embodiments. However, the foregoing description is not intended to limit the invention to the exemplified embodiments, and the skilled artisan should recognize that variations can be made within the spirit and scope of the invention as described in the foregoing specification. 

1. A conjugate having a structure encompassed by the formula:

wherein: R is a residue of a polyol, polythiol or polyamine bearing at from 3 to about 50 hydroxyl, thiol or amino groups; each R¹ is H or methyl; each R² is H or methyl, with the provisio that R¹ and R² are not both H; each Q is a linker; each POLY¹ is a water-soluble, non-peptidic polymer; each X is spacer moiety; and q is a positive integer from 3 to about 50, and pharmaceutically acceptable salts and solvates thereof.
 2. The conjugate of claim 1, wherein R is a residue of a polyol.
 3. The conjugate of claim 2, wherein the polyol is selected from the group consisting of glycerol, diglycerol, triglycerol, hexaglycerol, trimethylolpropane, pentaerythritol, dipentaerythritol, tripentaerythritol and ethoxylated forms of glycerol.
 4. The conjugate of claim 3, wherein the polyol is pentaerythritol.
 5. The conjugate of claim 1, wherein the linker is selected from the group consisting of —O—, —S—, —NH—, —NH—C(O)—, and —C(O)—NH—.
 6. The conjugate of claim 8, wherein the linker is —O—.
 7. The conjugate of claim 1, wherein the water-soluble, non-peptidic polymer is a poly(ethylene oxide).
 8. The conjugate of claim 1, wherein the spacer moiety has the structure corresponding to —Y—Z—, where Y is —(CR_(x),R_(y))_(a)—K_(w)—(CR_(x)R_(y))_(b)—(CH₂CH₂O)_(c)—(CR_(x)R_(y))_(d)—K_(z)—, wherein: each R_(x) and R_(y), in each occurrence, is independently H or an organic radical selected from the group consisting of alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, and substituted aryl, (a) is zero or an integer of from 1 to 12; (b) is zero or an integer of from 1 to 12; (c) is zero or an integer of from 1 to 25; (d) is zero or an integer of from 1 to 12; K_(w) is selected from the group consisting of —NH—, —C(O)—, —C(O)NH—, —NH—C(O)—, —O—, —S—, O—C(O)—, C(O)—O—, —O—C(O)—O—, O—C(O)—NH—, —NH—C(O)—O—; and K_(z) is selected from the group consisting of —NH—, —C(O)—, —C(O)NH—, —NH—C(O)—, —O—, —S—, O—C(O)—, C(O)—O—, —O—C(O)—O—, O—C(O)—NH—, —NH—C(O)—O—, and Z is selected from —C(O)—O—, O—C(O)—O—, —O—C(O)—NH— and —NH—C(O)—O—.
 9. The conjugate of claim 1, wherein the spacer moiety has the structure corresponding to —Y—, where Y is —(CR_(x),R_(y))_(a)—K_(w)—(CR_(x)R_(y))_(b)—(CH₂CH₂O)_(c)—(CR_(x)R_(y))_(d)—K_(z)—, wherein: each R_(x) and R_(y), in each occurrence, is independently H or an organic radical selected from the group consisting of alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, and substituted aryl, (a) is zero or an integer of from 1 to 12; (b) is zero or an integer of from 1 to 12; (c) is zero or an integer of from 1 to 25; (d) is zero or an integer of from 1 to 12; K_(w) is selected from the group consisting of —NH—, —C(O)—, —C(O)NH—, —NH—C(O)—, —O—, —S—, O—C(O)—, C(O)—O—, —O—C(O)—O—, O—C(O)—NH—, —NH—C(O)—O—; and K_(z) is selected from the group consisting of —NH—, —C(O)—, —C(O)NH—, —NH—C(O)—, —O—, —S—, O—C(O)—, C(O)—O—, —O—C(O)—O—, O—C(O)—NH—, —NH—C(O)—O—.
 10. The conjugate of claim 1, wherein the spacer moiety has the structure C(O)—(CH₂)_(b)—(CH₂CH₂O)_(c)—(CH₂)_(d)—K_(z)—, wherein: (b) is an integer of from 1 to 5; (c) is an integer of from 1 to 5; (d) is an integer of from 1 to 5; and K_(z) is selected from the group consisting of —NH—, —C(O)—, —C(O)NH—, —NH—C(O)—, —O—, —S—, O—C(O)—, C(O)—O—, —O—C(O)—O—, O—C(O)—NH—, —NH—C(O)—O—.
 11. The conjugate of claim 1, wherein the spacer moiety has the structure —[CH₂]_(m)— or —CH₂—C(O)—NH—C(R⁴)H—, wherein m is a positive integer and R⁴ is selected from the group consisting of H, CH₃, CH(CH₃)₂, CH₂CH(CH₃)₂ and C(H)(CH₃)CH₂CH₃.
 12. The conjugate of claim 1, wherein “q” is an integer from 3 to
 10. 13. The conjugate of claim 1, wherein each R¹ is methyl and each R² is methyl.
 14. The conjugate of claim 1, wherein each R¹ is H and each R² is methyl.
 15. The conjugate of claim 1, wherein each R¹ is methyl and each R² is H.
 16. A conjugate having a structure encompassed by the formula:

wherein: each R³ is selected from the group consisting of H, CH₃, CH(CH₃)₂, CH₂CH(CH₃)₂ and C(H)(CH₃)CH₂CH₃; each n is a positive integer from 10 to about 400; and pharmaceutically acceptable salts and solvates thereof.
 17. A conjugate having a structure encompassed by the formula:

wherein: each R¹ is H or methyl; each R² is H or methyl, with the provisio that R¹ and R² are not both H; each n is a positive integer from 10 to about 400; each m is a positive integer from 1 to about 12; and and pharmaceutically acceptable salts and solvates thereof.
 18. A conjugate having a structure encompassed by the formula:

wherein: each R¹ is H or methyl; each R² is H or methyl, with the provisio that R¹ and R² are not both H; each n is a positive integer from 10 to about 400; each L¹ is —(CH₂)₀₋₆C(═O)—; each L² is —NH(CH₂CH₂O)₁₋₁₀(CHR⁵)₁₋₆—, wherein each R⁵ is independently selected from the group consisting of H and lower alkyl; and q is a positive integer from 3 to about 50, and pharmaceutically acceptable salts and solvates thereof.
 19. A conjugate having a structure encompassed by the formula:

wherein: each R¹ is H or methyl; each R² is H or methyl, with the provisio that R¹ and R² are not both H; each n is a positive integer from 10 to about 400; each L¹ is —(CH₂)₀₋₆C(═O)—; and each L³ is —[NH(CHR⁵)₁₋₆C(═O)]₁₋₄—, wherein each R⁵ is independently selected from the group consisting of H and lower alkyl; and q is a positive integer from 3 to about 50, and pharmaceutically acceptable salts and solvates thereof.
 20. A conjugate having a structure encompassed by the formula:

wherein: each R¹ is H or methyl; each R² is H or methyl, with the provisio that R¹ and R² are not both H; each n is a positive integer from 10 to about 400; each L¹ is —C(═O)—; each L³ is —[NH(CHR⁵)₁₋₆C(═O)]₁₋₄—, wherein each R⁵ is independently selected from the group consisting of H and lower alkyl; and q is a positive integer from 3 to about 50, and pharmaceutically acceptable salts and solvates thereof.
 21. A conjugate selected from the group consisting of

wherein each n is a positive integer from 10 to about 400, and pharmaceutically acceptable salts of each of the foregoing.
 22. A composition comprising a plurality of conjugates, wherein at least 80% of the conjugates in the composition have a structure encompassed by claim
 1. 23. The composition of claim 22, further comprising a pharmaceutical excipient. 